Ciuffetti G, Mercuri M, Mannarino E, Lombardini R, Pasqualini L, Ott C, Lupattelli G
2nd Department of Internal Medicine, University of Perugia, Italy.
Eur J Clin Invest. 1991 Feb;21(1):111-7. doi: 10.1111/j.1365-2362.1991.tb01367.x.
Leucocyte-derived free radicals were monitored in 30 stage II peripheral vascular disease (PVD) patients in an open placebo-controlled study. Linked to a transcutaneous oxygen pressure (TcPO2) monitor, they performed two consecutive standard treadmill tests (5 min, 2 km h-1, 12% slope) before and after 15-days treatment with placebo or a leucocyte-derived free radical scavenger (Piroxicam, 20 mg day-1), the second test being carried out at the TcPO2 half-recovery time. Blood samples were collected at baseline, at the maximum walking times and the TcPO2 half recovery times. The total and differential leucocyte counts, the percentage of cells with pseudopodia or cytoplasmatic irregularities, the filterability rates (using a positive pressure Nuclepore filter system) of the main leucocyte subfractions and plasma oxidant activity were monitored. Compared with values before treatment and with the placebo-treated group Piroxicam therapy significantly (P less than 0.001) reduced the final half-recovery time, the percentage of cells with pseudopodia and the level of plasma oxidant activity (P less than 0.01) and kept the granulocyte filterability rate stable, showing leucocyte-derived free radicals are involved in peripheral ischaemia.
在一项开放性安慰剂对照研究中，对30例II期周围血管疾病（PVD）患者的白细胞衍生自由基进行了监测。将其与经皮氧分压（TcPO2）监测仪相连，患者在接受安慰剂或白细胞衍生自由基清除剂（吡罗昔康，每日20 mg）治疗15天前后，连续进行两次标准跑步机测试（5分钟，2公里/小时，12%坡度），第二次测试在TcPO2半恢复时间进行。在基线、最大步行时间和TcPO2半恢复时间采集血样。监测白细胞总数及分类计数、有伪足或细胞质不规则的细胞百分比、主要白细胞亚群的过滤率（使用正压核孔过滤系统）以及血浆氧化活性。与治疗前的值和安慰剂治疗组相比，吡罗昔康治疗显著（P<0.001）缩短了最终半恢复时间、有伪足的细胞百分比和血浆氧化活性水平（P<0.01），并使粒细胞过滤率保持稳定，表明白细胞衍生自由基参与了周围缺血。
