通过创建与固相偶联的可重复使用的第一链cDNA库提高单链PCR效率。 - Suppr

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超能文献

Improved efficiency for single-sided PCR by creating a reusable pool of first-strand cDNA coupled to a solid phase.

作者信息

Raineri I, Moroni C, Senn H P

机构信息

Institut für Medizinische Mikrobiologie, Universität Basel, Switzerland.

出版信息

Nucleic Acids Res. 1991 Jul 25;19(14):4010. doi: 10.1093/nar/19.14.4010.

DOI:10.1093/nar/19.14.4010

PMID:1862000

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328509/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c16/328509/004a9ec57e23/nar00094-0211-a.jpg

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本文引用的文献

Structure of the chromosomal gene for murine interleukin 3.小鼠白细胞介素3染色体基因的结构

Proc Natl Acad Sci U S A. 1985 Jan;82(2):316-20. doi: 10.1073/pnas.82.2.316.

Human N-ras: cDNA cloning and gene structure.人类N-ras：cDNA克隆与基因结构。

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Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.从稀有转录本快速生成全长cDNA：使用单一基因特异性寡核苷酸引物进行扩增

Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002. doi: 10.1073/pnas.85.23.8998.

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