• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Improved efficiency for single-sided PCR by creating a reusable pool of first-strand cDNA coupled to a solid phase.

作者信息

Raineri I, Moroni C, Senn H P

机构信息

Institut für Medizinische Mikrobiologie, Universität Basel, Switzerland.

出版信息

Nucleic Acids Res. 1991 Jul 25;19(14):4010. doi: 10.1093/nar/19.14.4010.

DOI:10.1093/nar/19.14.4010
PMID:1862000
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328509/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c16/328509/004a9ec57e23/nar00094-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c16/328509/004a9ec57e23/nar00094-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c16/328509/004a9ec57e23/nar00094-0211-a.jpg

相似文献

1
Improved efficiency for single-sided PCR by creating a reusable pool of first-strand cDNA coupled to a solid phase.通过创建与固相偶联的可重复使用的第一链cDNA库提高单链PCR效率。
Nucleic Acids Res. 1991 Jul 25;19(14):4010. doi: 10.1093/nar/19.14.4010.
2
Solid-phase reversible immobilization for the isolation of PCR products.用于分离PCR产物的固相可逆固定化。
Nucleic Acids Res. 1995 Nov 25;23(22):4742-3. doi: 10.1093/nar/23.22.4742.
3
PCR: catching the next wave.
Biotechnology (N Y). 1992 Feb;10(2):137-40.
4
Saturation mutagenesis by mutagenic oligonucleotide-directed PCR amplification (Mod-PCR).通过诱变寡核苷酸定向PCR扩增进行饱和诱变（Mod-PCR）。
Methods Mol Biol. 1996;57:311-21. doi: 10.1385/0-89603-332-5:311.
5
Cloning and direct sequencing of plant promoters using primer-adapter mediated PCR on DNA coupled to a magnetic solid phase.利用与磁性固相偶联的DNA上的引物-衔接子介导PCR对植物启动子进行克隆和直接测序
Biotechniques. 1992 Jul;13(1):74-81.
6
Construction of chimeric molecules by a two-step recombinant PCR method.
Biotechniques. 1994 Jun;16(6):1010-1.
7
Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion.蛋白酶K消化后聚合酶链反应（PCR）产物的克隆效率提高。
Nucleic Acids Res. 1991 Jan 11;19(1):184. doi: 10.1093/nar/19.1.184.
8
Reusable cDNA libraries coupled to magnetic beads.与磁珠偶联的可重复使用的互补DNA文库。
Anal Biochem. 1992 Oct;206(1):206-7. doi: 10.1016/s0003-2697(05)80035-1.
9
Molecular mechanism controlling the incorporation of fluorescent nucleotides into DNA by PCR.通过聚合酶链式反应（PCR）控制荧光核苷酸掺入DNA的分子机制。
Cytometry. 1997 Jul 1;28(3):206-11.
10
Simultaneous amplification of 5' and 3' cDNA ends based on template-switching effect and inverse PCR.基于模板转换效应和反向PCR同时扩增5'和3' cDNA末端
Biotechniques. 2006 Feb;40(2):187-9. doi: 10.2144/000112051.

引用本文的文献

1
RNA-seq profiling of primary calli induced by different media and photoperiods for rice 'Yunyin'.不同培养基和光周期诱导水稻‘云引’形成的愈伤组织的RNA测序分析
Mol Breed. 2022 Mar 7;42(3):13. doi: 10.1007/s11032-022-01283-y. eCollection 2022 Mar.
2
Quantitative analysis of gene expression in a single cell by qPCR.通过定量聚合酶链反应对单个细胞中的基因表达进行定量分析。
Nat Methods. 2009 Jul;6(7):503-6. doi: 10.1038/nmeth.1338. Epub 2009 Jun 14.
3
Optimization of RNA isolation from Brittle Leaf Disease affected date palm leaves and construction of a subtractive cDNA library.

本文引用的文献

1
Structure of the chromosomal gene for murine interleukin 3.小鼠白细胞介素3染色体基因的结构
Proc Natl Acad Sci U S A. 1985 Jan;82(2):316-20. doi: 10.1073/pnas.82.2.316.
2
Human N-ras: cDNA cloning and gene structure.人类N-ras：cDNA克隆与基因结构。
Nucleic Acids Res. 1985 Jul 25;13(14):5255-68. doi: 10.1093/nar/13.14.5255.
3
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.从稀有转录本快速生成全长cDNA：使用单一基因特异性寡核苷酸引物进行扩增
从受脆叶病影响的海枣叶片中优化RNA提取及构建消减cDNA文库
Mol Biotechnol. 2009 Jan;41(1):63-8. doi: 10.1007/s12033-008-9104-1. Epub 2008 Sep 25.
4
A simple semisolid subtraction method using carbodiimide-coated microplates.一种使用碳二亚胺包被微孔板的简单半固体减法方法。
Mol Biotechnol. 2000 Jul;15(3):193-200. doi: 10.1385/MB:15:3:193.
5
Subtractive hybridization of cDNA from small amounts of plant tissue.从少量植物组织中进行cDNA消减杂交。
Mol Biotechnol. 1997 Aug;8(1):7-12. doi: 10.1007/BF02762335.
6
Directional random oligonucleotide primed (DROP) global amplification of cDNA: its application to subtractive cDNA cloning.定向随机寡核苷酸引物介导的（DROP）cDNA全基因组扩增：其在消减cDNA克隆中的应用
Nucleic Acids Res. 1996 Dec 1;24(23):4832-5. doi: 10.1093/nar/24.23.4832.
7
AUH, a gene encoding an AU-specific RNA binding protein with intrinsic enoyl-CoA hydratase activity.AUH，一种编码具有内在烯酰辅酶A水合酶活性的AU特异性RNA结合蛋白的基因。
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2051-5. doi: 10.1073/pnas.92.6.2051.
8
cDNA library construction from small amounts of RNA using paramagnetic beads and PCR.使用顺磁珠和聚合酶链反应从少量RNA构建互补DNA文库。
Nucleic Acids Res. 1993 Feb 11;21(3):775-6. doi: 10.1093/nar/21.3.775.
9
Isolation and characterization of the EF-1 alpha gene of the filamentous fungus Puccinia graminis f. sp. tritici.禾本科柄锈菌小麦专化型丝状真菌EF-1α基因的分离与鉴定
Curr Genet. 1995 Mar;27(4):367-72. doi: 10.1007/BF00352106.
10
Analysis of human VH gene repertoire expression in peripheral CD19+ B cells.外周血CD19⁺ B细胞中人VH基因库表达分析
Immunogenetics. 1995;42(5):342-52. doi: 10.1007/BF00179395.
Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002. doi: 10.1073/pnas.85.23.8998.