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人类N-ras：cDNA克隆与基因结构。

Human N-ras: cDNA cloning and gene structure.

作者信息

Hall A, Brown R

出版信息

Nucleic Acids Res. 1985 Jul 25;13(14):5255-68. doi: 10.1093/nar/13.14.5255.

DOI:10.1093/nar/13.14.5255
PMID:2991860
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321863/
Abstract

The structure and organisation of the human N-ras gene has been determined by analysing cDNA clones derived from the two main mRNA transcripts. One clone in particular is 4.1 Kb long and originates from the larger (4.3 Kb) message. Sequence analysis of this clone has revealed that the N-ras gene consists of seven exons. A second clone deriving from the smaller (2 Kb) message shows that the difference between the two transcripts is a simple extension through the termination site of the 2 Kb transcript. Using S1 analysis, two transcriptional starts have been mapped, 10 bp apart. There is no obvious TATA box in the expected promoter region of the gene, though there are 4 GGGCGG sequences surrounding the start sites. The 5' untranslated sequence contains 2 ATGs upstream of the initiation codon.

摘要

通过分析源自两种主要mRNA转录本的cDNA克隆，已确定了人类N-ras基因的结构和组织。特别是一个克隆长4.1 kb，源自较大的（4.3 kb）信使RNA。对该克隆的序列分析表明，N-ras基因由七个外显子组成。源自较小（2 kb）信使RNA的第二个克隆表明，两种转录本之间的差异是通过2 kb转录本的终止位点的简单延伸。使用S1分析，已定位了两个转录起始点，相距10 bp。在该基因预期的启动子区域中没有明显的TATA框，尽管在起始位点周围有4个GGGCGG序列。5'非翻译序列在起始密码子上游包含2个ATG。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b77/321863/935247f80ddd/nar00308-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b77/321863/a2b8e5320744/nar00308-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b77/321863/935247f80ddd/nar00308-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b77/321863/a2b8e5320744/nar00308-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b77/321863/935247f80ddd/nar00308-0257-a.jpg

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