Fukuwatari Tsutomu, Shibata Katsumi
Department of Food Science and Nutrition, School of Human Cultures, The University of Shiga Prefecture, Hikone, Shiga, Japan.
J Nutr Sci Vitaminol (Tokyo). 2008 Jun;54(3):223-9. doi: 10.3177/jnsv.54.223.
Little information is available to estimate water-soluble vitamin intakes from urinary vitamins and their metabolite contents as possible nutritional markers. Determination of the relationships between the oral dose and urinary excretion of water-soluble vitamins in human subjects contributes to finding valid nutrition markers of water-soluble vitamin intakes. Six female Japanese college students were given a standard Japanese diet in the first week, the same diet with a synthesized water-soluble vitamin mixture as a diet with approximately onefold vitamin mixture based on Dietary Reference Intakes (DRIs) for Japanese in the second week, with a threefold vitamin mixture in the third week, and a sixfold mixture in the fourth week. Water-soluble vitamins and their metabolites were measured in the 24-h urine collected each week. All urinary vitamins and their metabolite levels except vitamin B(12) increased linearly in a dose-dependent manner, and highly correlated with vitamin intake (r=0.959 for vitamin B(1), r=0.927 for vitamin B(2), r=0.965 for vitamin B(6), r=0.957 for niacin, r=0.934 for pantothenic acid, r=0.907 for folic acid, r=0.962 for biotin, and r=0.952 for vitamin C). These results suggest that measuring urinary water-soluble vitamins and their metabolite levels can be used as good nutritional markers for assessing vitamin intakes.
目前几乎没有信息可用于根据尿中维生素及其代谢物含量来估计水溶性维生素摄入量，以此作为可能的营养指标。确定人体受试者口服剂量与水溶性维生素尿排泄之间的关系，有助于找到有效的水溶性维生素摄入量营养指标。六名日本女大学生在第一周食用标准日本饮食，第二周食用添加了合成水溶性维生素混合物的相同饮食，该混合物的维生素含量约为日本膳食营养素参考摄入量（DRIs）的一倍，第三周食用三倍维生素混合物，第四周食用六倍维生素混合物。每周收集的24小时尿液中测量水溶性维生素及其代谢物。除维生素B12外，所有尿中维生素及其代谢物水平均呈剂量依赖性线性增加，且与维生素摄入量高度相关（维生素B1的r=0.959，维生素B2的r=0.927，维生素B6的r=0.965，烟酸的r=0.957，泛酸的r=0.934，叶酸的r=0.907，生物素的r=0.962，维生素C的r=0.952）。这些结果表明，测量尿中水溶性维生素及其代谢物水平可作为评估维生素摄入量的良好营养指标。
