Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with HbD: a study of 129 samples.
作者信息
Moiz Bushra, Hashmi Mashhooda Rasool, Sadaf Shahmina
机构信息
Department of Pathology and Microbiology, The Aga Khan University, Karachi, Pakistan.
出版信息
Clin Biochem. 2008 Oct;41(14-15):1204-10. doi: 10.1016/j.clinbiochem.2008.06.015. Epub 2008 Jul 3.
OBJECTIVES
To compare ion exchange and boronate affinity chromatography for HbA(1c) estimation in patients with type I and II diabetes having hemoglobin D.
DESIGN AND METHODS
Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA(1c) estimation in patients with homozygous and heterozygous D disease.
RESULTS
Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA(1c) estimation.
CONCLUSIONS
The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.
Zotero 插件