Ruizeveld de Winter J A, Trapman J, Vermey M, Mulder E, Zegers N D, van der Kwast T H
Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
J Histochem Cytochem. 1991 Jul;39(7):927-36. doi: 10.1177/39.7.1865110.
The cellular localization of the human androgen receptor was visualized immunohistochemically using a mouse monoclonal antibody (MAb) F39.4, directed against a fragment of the N-terminal domain of the androgen receptor. The nuclear immunoreactivity of various human tissues with F39.4 was generally consistent with earlier biochemical and autoradiographic data. However, previously suggested androgen receptor expression in thyroid, pancreatic, gastrointestinal, and bladder tissues was not confirmed immunohistochemically. Stratified squamous epithelia of vagina and cervix showed selective immunostaining of the basal cell layer, whereas in the preputial epithelium the intensity of immunoreactivity decreased gradually with maturation. In contrast, glandular epithelia of the sweat glands, male accessory sex organs, and female breast showed nearly exclusive F39.4 staining of the inner cylindric layer. In the testis, Sertoli cells, peritubular myoid cells, and interstitial cells were immunoreactive with MAb F39.4. Expression of the androgen receptor by smooth muscle tissue was largely confined to the male reproductive organs. The specificity and sensitivity of this simple and rapidly performed immunohistochemical technique in the detection of the human androgen receptor at the cellular and subcellular level makes it worthwhile to study tissue androgen receptor expression by immunohistochemistry in physiological and pathological states.
使用针对雄激素受体N端结构域片段的小鼠单克隆抗体（MAb）F39.4，通过免疫组织化学方法观察人雄激素受体的细胞定位。F39.4对各种人体组织的核免疫反应性总体上与早期的生化和放射自显影数据一致。然而，先前提示的甲状腺、胰腺、胃肠道和膀胱组织中的雄激素受体表达未得到免疫组织化学证实。阴道和宫颈的复层鳞状上皮显示基底层有选择性免疫染色，而在包皮上皮中，免疫反应强度随成熟逐渐降低。相反，汗腺、男性附属生殖器官和女性乳腺的腺上皮显示内圆柱层几乎完全被F39.4染色。在睾丸中，支持细胞、睾丸肌样细胞和间质细胞对MAb F39.4有免疫反应性。平滑肌组织中雄激素受体的表达主要局限于男性生殖器官。这种简单且操作迅速的免疫组织化学技术在细胞和亚细胞水平检测人雄激素受体的特异性和敏感性，使得通过免疫组织化学研究生理和病理状态下组织雄激素受体表达具有价值。
