Takeda H, Chodak G, Mutchnik S, Nakamoto T, Chang C
Department of Surgery/Urology, University of Chicago, Illinois 60637.
J Endocrinol. 1990 Jul;126(1):17-25. doi: 10.1677/joe.0.1260017.
Rat, human, and mouse tissues were stained immunohistochemically using mono- and polyclonal androgen receptor antibodies. Monoclonal antibodies were raised in rats and used to stain human and mouse tissues; polyclonal antibodies were raised in rabbits and used to stain rat tissues. Frozen tissue sections were incubated with the appropriate androgen receptor antibody and staining was completed by the indirect avidin-biotin peroxidase method. A comprehensive survey of rat and mouse tissues was performed. Antibody staining was found exclusively in the nucleus of certain specific cell types, suggesting that the androgen receptor is a nuclear protein. All male sexual organs in the rat showed strong positive nuclear staining for androgen receptor. Weaker positive reactions were seen in kidney, liver, adrenal cortex and pituitary gland. Furthermore, positive staining for androgen receptor was exhibited in skeletal, cardiac and smooth muscle cells, and central nervous tissue. Female reproductive organs also contained androgen receptor-positive cells. The spleen was found to be the only organ examined which did not stain for androgen receptor. The monoclonal antibody could also demonstrate androgen receptor-positive cells in a human prostatic cancer and in a prostate with benign hyperplasia. These data demonstrate the use of antibodies in revealing cellular/subcellular distribution of androgen receptor in target tissues.
使用单克隆和多克隆雄激素受体抗体对大鼠、人类和小鼠组织进行免疫组织化学染色。单克隆抗体在大鼠体内产生，用于对人类和小鼠组织进行染色；多克隆抗体在兔子体内产生，用于对大鼠组织进行染色。将冷冻组织切片与适当的雄激素受体抗体孵育，并用间接抗生物素蛋白-生物素过氧化物酶法完成染色。对大鼠和小鼠组织进行了全面的调查。抗体染色仅在某些特定细胞类型的细胞核中发现，这表明雄激素受体是一种核蛋白。大鼠的所有雄性性器官对雄激素受体均显示出强烈的阳性核染色。在肾脏、肝脏、肾上腺皮质和垂体中可见较弱的阳性反应。此外，在骨骼肌、心肌和平滑肌细胞以及中枢神经组织中也表现出雄激素受体的阳性染色。雌性生殖器官也含有雄激素受体阳性细胞。发现脾脏是所检查的唯一未对雄激素受体染色的器官。该单克隆抗体还可在人类前列腺癌和良性增生前列腺中显示雄激素受体阳性细胞。这些数据证明了抗体在揭示雄激素受体在靶组织中的细胞/亚细胞分布方面的用途。
