Institute of Bioengineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2011 Feb;102(4):3923-6. doi: 10.1016/j.biortech.2010.11.112. Epub 2010 Nov 28.
Butyric acid is an important specialty chemical with wide industrial applications. The feasible large-scale fermentation for the economical production of butyric acid requires low-cost substrate and efficient process. In the present study, butyric acid production by immobilized Clostridium tyrobutyricum was successfully performed in a fibrous-bed bioreactor using Jerusalem artichoke as the substrate. Repeated-batch fermentation was carried out to produce butyric acid with a high butyrate yield (0.44 g/g), high productivity (2.75 g/L/h) and a butyrate concentration of 27.5 g/L. Furthermore, fed-batch fermentation using sulfuric acid pretreated Jerusalem artichoke hydrolysate resulted in a high butyric acid concentration of 60.4 g/L, with the yield of 0.38 g/g and the selectivity of ∼ 85.1 (85.1g butyric acid/g acetic acid). Thus, the production of butyric acid from Jerusalem artichoke on a commercial scale could be achieved based on the system developed in this work.
丁酸是一种重要的精细化学品，具有广泛的工业应用。经济生产丁酸的可行的大规模发酵需要低成本的基质和高效的工艺。在本研究中，使用固定化酪丁酸梭菌在纤维床生物反应器中成功地使用菊芋作为基质生产丁酸。进行了分批补料发酵，以生产丁酸，丁酸产量（0.44g/g）高，生产强度（2.75g/L/h）高，丁酸浓度为 27.5g/L。此外，使用硫酸预处理的菊芋水解物进行分批补料发酵，丁酸浓度高达 60.4g/L，产率为 0.38g/g，选择性约为 85.1（85.1g 丁酸/g 乙酸）。因此，基于本工作中开发的系统，可以实现菊芋的商业规模丁酸生产。
