Westh H, Jensen J S
Department of Clinical Microbiology, Hvidovre Hospital, Kettegård Alle 30, DK-2650 Hvidovre, Denmark.
Sex Transm Infect. 2008 Dec;84(7):546-7. doi: 10.1136/sti.2008.031906. Epub 2008 Jul 24.
We estimated the prevalence of the new variant of Chlamydia trachomatis in Denmark. Fifty consecutive C trachomatis urine samples from each of the 14 regional microbiology laboratories were initially tested and re-analysed with a real-time C trachomatis PCR assay targeting the 16S rRNA gene and with the Becton Dickinson ProbeTec with confirmation of positives with a plasmid PCR with the primers CTP200/CTP201 flanking the deletion. The positive rate for 691 samples was 17% by real-time C trachomatis PCR and by ProbeTec. One specimen was positive for the new variant of C trachomatis (0.14% of all samples; 0.8% of the positives). The prevalence of the new variant of C trachomatis is very low despite very close contact between Denmark and Sweden and extensive cross-border commuting.
我们估计了丹麦沙眼衣原体新变体的流行率。从14个地区微生物实验室中各选取50份连续的沙眼衣原体尿液样本,首先使用针对16S rRNA基因的实时沙眼衣原体PCR检测法进行检测,并使用Becton Dickinson ProbeTec进行重新分析,通过用位于缺失侧翼的引物CTP200/CTP201进行质粒PCR来确认阳性结果。691份样本通过实时沙眼衣原体PCR和ProbeTec检测的阳性率为17%。有1份标本沙眼衣原体新变体呈阳性(占所有样本的0.14%;占阳性样本的0.8%)。尽管丹麦和瑞典之间联系紧密且存在广泛的跨境通勤,但沙眼衣原体新变体的流行率非常低。