Gross Gabriele, van der Meulen Jan, Snel Johannes, van der Meer Roelof, Kleerebezem Michiel, Niewold Theo A, Hulst Marcel M, Smits Mari A
Animal Breeding and Genomics Centre, Animal Sciences Group of Wageningen UR, Lelystad, The Netherlands.
FEMS Immunol Med Microbiol. 2008 Nov;54(2):215-23. doi: 10.1111/j.1574-695X.2008.00466.x. Epub 2008 Jul 30.
Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and msa-mutant strain had been applied. In contrast to the mutant strains, the L. plantarum wild-type strain tended to induce a decrease in jejunal net fluid absorption compared with control conditions. Furthermore, after 8 h of perfusion expression of the host gene encoding pancreatitis-associated protein, a protein with proposed bactericidal properties, was found to be upregulated by the wild-type strain only. These observations suggest a role of Msa in the induction of host responses in the pig intestine.
肠道中的宿主-微生物相互作用十分复杂，对于引发肠道中宿主反应的特定非致病性微生物因子，人们了解甚少。在本研究中，使用原位猪小肠段灌注模型，研究了植物乳杆菌299v与空肠上皮细胞的甘露糖特异性相互作用。将植物乳杆菌299v野生型菌株的作用与两种相应突变菌株进行了比较，这两种突变菌株分别缺失编码甘露糖特异性粘附素（msa）的基因或分选酶（srtA；负责将细胞表面蛋白如Msa锚定到细胞壁）。当应用野生型和msa突变型菌株的混合物时，灌注8小时后可观察到与肠表面相关的野生型菌株略有富集。与突变菌株相比，植物乳杆菌野生型菌株与对照条件相比倾向于诱导空肠净液体吸收减少。此外，灌注8小时后，发现仅野生型菌株上调了编码胰腺炎相关蛋白（一种具有推测杀菌特性的蛋白）的宿主基因的表达。这些观察结果表明Msa在猪肠道宿主反应诱导中发挥作用。
