Chen Han-Qing, Wang Xi-Jin, Jin Zheng-Yu, Xu Xue-Ming, Zhao Jian-Wei, Xie Zheng-Jun
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Neurosci Res. 2008 Oct;62(2):123-30. doi: 10.1016/j.neures.2008.07.001. Epub 2008 Jul 16.
In the present study, protective effect of five isoflavones (formononetin, daidzein, pratensein, calycosin and irilone) from Trifolium pratense on lipopolysaccharide-induced dopaminergic neurodegeneration was studied for the first time. The results showed that all five isoflavones attenuated LPS-induced decrease in dopamine uptake and the number of dopaminergic neurons in a dose-dependent manner in rat mesencephalic neuron-glia cultures. Moreover, they also significantly inhibited LPS-induced activation of microglia and production of tumor necrosis factor-alpha, nitric oxide and superoxide in mesencephalic neuron-glia cultures and microglia-enriched cultures. In addition, the rank order of protective potency of five isoflavones was: pratensein>daidzein>calycosin>formononetin>irilone. This study suggested that all five isoflavones protected dopaminergic neurons against LPS-induced injury through inhibition of microglia activation and proinflammatory factors generation.
在本研究中，首次对红车轴草中的五种异黄酮（芒柄花黄素、大豆苷元、草原黄芩素、毛蕊异黄酮和鸢尾黄酮）对脂多糖诱导的多巴胺能神经变性的保护作用进行了研究。结果表明，在大鼠中脑神经元-胶质细胞培养物中，所有五种异黄酮均以剂量依赖的方式减轻了脂多糖诱导的多巴胺摄取减少和多巴胺能神经元数量的减少。此外，它们还显著抑制了脂多糖诱导的中脑神经元-胶质细胞培养物和富含小胶质细胞的培养物中小胶质细胞的活化以及肿瘤坏死因子-α、一氧化氮和超氧化物的产生。此外，五种异黄酮的保护效力排序为：草原黄芩素>大豆苷元>毛蕊异黄酮>芒柄花黄素>鸢尾黄酮。本研究表明，所有五种异黄酮均通过抑制小胶质细胞活化和促炎因子生成来保护多巴胺能神经元免受脂多糖诱导的损伤。
