Koca Suleyman Serdar, Ozgen Metin, Aydin Suleyman, Dag Sait, Evren Bahri, Isik Ahmet
Department of Rheumatology, Faculty of Medicine, Firat University, Firat Tip Merkezi, Ic Hastaliklari AD./Romatoloji BD., 23119 Elazig, Turkey.
Inflammation. 2008 Oct;31(5):329-35. doi: 10.1007/s10753-008-9082-2.
Ghrelin is a powerful, endogenous orexigenic peptide. In addition, ghrelin has anti-inflammatory effects, and it has been reported that ghrelin down-regulates pro-inflammatory cytokines, including interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha. Obestatin appears to decrease food intake and appetite, and its potential role in inflammation is not yet clear. The aims of this study were to assess total and acylated (active) ghrelin and obestatin serum levels and their relations with inflammatory status in rheumatoid arthritis (RA) patients.
Fasting blood samples were obtained from 37 patients with RA, 29 patients with Behçet's disease (BD) and 28 healthy controls (HC). Total ghrelin and obestatin levels were measured by radioimmunoassay and acylated ghrelin was quantified by enzyme-linked immunosorbent assay.
Patients with RA had lower total ghrelin, but higher obestatin levels than patients with BD (p<0.05 for both), but when compared with HC group differences were not significant. There was no difference across groups in terms of acylated ghrelin. Total ghrelin level was not correlated with any study parameters in the all groups. Obestatin level correlated with erythrocyte sedimentation rate and DAS-28 in the RA group, the level of IL-6 in the BD group, and with the level of TNF-alpha in the HC group (r=0.400, p<0.05; r=0.412, p<0.05, r=0.543, p<0.01 and r=0.528, p<0.05, respectively).
Our results did not show a significant correlation between circulating ghrelin and clinical or laboratory markers of disease activity in RA. Surprisingly, obestatin correlated with some inflammatory markers. So, obestatin seems to be more valuable than ghrelin in the pathogenesis of RA.
胃饥饿素是一种强效的内源性促食欲肽。此外，胃饥饿素具有抗炎作用，据报道胃饥饿素可下调促炎细胞因子，包括白细胞介素（IL）-1β和肿瘤坏死因子（TNF）-α。肥胖抑制素似乎可减少食物摄入量和食欲，其在炎症中的潜在作用尚不清楚。本研究的目的是评估类风湿关节炎（RA）患者血清中总胃饥饿素和酰化（活性）胃饥饿素以及肥胖抑制素的水平及其与炎症状态的关系。
采集37例RA患者、29例白塞病（BD）患者和28例健康对照者（HC）的空腹血样。采用放射免疫分析法测定总胃饥饿素和肥胖抑制素水平，采用酶联免疫吸附测定法定量酰化胃饥饿素。
RA患者的总胃饥饿素水平低于BD患者，但肥胖抑制素水平高于BD患者（两者p均<0.05），但与HC组相比差异无统计学意义。各组间酰化胃饥饿素水平无差异。所有组中总胃饥饿素水平与任何研究参数均无相关性。在RA组中，肥胖抑制素水平与红细胞沉降率和疾病活动评分28（DAS-28）相关，在BD组中与IL-6水平相关，在HC组中与TNF-α水平相关（r分别为0.400，p<0.05；r为0.412，p<0.05；r为0.543，p<0.01；r为0.528，p<0.05）。
我们的结果未显示循环胃饥饿素与RA患者疾病活动的临床或实验室指标之间存在显著相关性。令人惊讶的是，肥胖抑制素与一些炎症标志物相关。因此，在RA的发病机制中，肥胖抑制素似乎比胃饥饿素更有价值。
