Sun Jianqiu, Guo Liangdong, Zang Wei, Ping Wenxiang, Chi Defu
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Sci China C Life Sci. 2008 Aug;51(8):751-9. doi: 10.1007/s11427-008-0091-z. Epub 2008 Aug 3.
A total of 973 isolates of endophytic fungi were recovered from 1,144 tissue fragments of the six medicinal plant species belonging to 4 families collected in the Beijing Botanical Garden. Of these isolates 778 sporulated and were identified into 21 taxa by morphological characteristics. Among the taxa 11 belonged to Coelomycetes, 6 to Ascomycetes, and 4 to Hyphomycetes. Various numbers of endophytic fungi (5-8 taxa) were obtained from each plant. Alternaria alternata was the dominant species in the 6 plants, and Microsphaeropsis conielloides was also dominant in Eucommia ulmoides. There were high colonization rates (47.9%-63.1%) and isolation rates (0.7-0.93) of endophytic fungi, and they were conspicuously higher in twigs than those in leaves in the 6 plants examined. The colonization and isolation rates of endophytic fungi increased with the twig age. The results based on the analyses of cluster and Sorenson's similarity coefficients indicated that some endophytic fungi showed a certain degree of host and tissue preference.
从北京植物园采集的隶属于4科的6种药用植物的1144个组织片段中，共分离得到973株内生真菌。其中778株产生孢子，并根据形态特征鉴定为21个分类单元。这些分类单元中，11个属于腔孢纲，6个属于子囊菌纲，4个属于丝孢纲。从每种植物中获得了不同数量的内生真菌（5 - 8个分类单元）。链格孢是这6种植物中的优势种，拟小卵孢在杜仲中也是优势种。内生真菌的定殖率（47.9% - 63.1%）和分离率（0.7 - 0.93）较高，在所检测的6种植物中，嫩枝中的定殖率和分离率明显高于叶片。内生真菌的定殖率和分离率随嫩枝年龄的增加而升高。基于聚类分析和索伦森相似系数的结果表明，一些内生真菌表现出一定程度的宿主和组织偏好。
