Lee Wi Young, Park Eung-Jun, Ahn Jin Kwon
Division of Biotechnology, Korea Forest Research Institute, Suwon, Republic of Korea.
Appl Biochem Biotechnol. 2009 Jul;158(1):213-21. doi: 10.1007/s12010-008-8322-0. Epub 2008 Aug 8.
The methods for increasing the production of ergothioneine (ERG) were investigated by using the mycelial culture of several mushroom species, primarily Ganoderma neo-japonicum. We first found that ERG was accumulated at the different levels in mycelia and fruiting bodies, respectively, depending on the mushroom species. As a result of adding various amino acids to the mycelial culture medium, methionine (Met) was shown to be the most effective additive. The most preferable condition of the additive was the combination of 4 mM Met and 1 g/l of yeast extract, and the maximum ERG production reached approximately 1.7 mg/l, which corresponds to 2.4 times (0.7 mg/l) that in the basal medium without Met. Although the supplementation of Met enhanced the ERG production, the mycelial growth was significantly inhibited. Furthermore, the analysis of amino acids in the culture medium revealed that the Met additive reduced the consumption rates of most amino acids tested, probably due to the decrease in mycelial growth. Taking these results into consideration, we suggest that the addition of Met to the mycelial culture medium is an efficient way to enhance the ERG production in economically important mushroom species.
通过使用几种蘑菇物种（主要是新日本灵芝）的菌丝体培养来研究提高麦角硫因（ERG）产量的方法。我们首先发现，根据蘑菇物种的不同，ERG分别在菌丝体和子实体中以不同水平积累。在向菌丝体培养基中添加各种氨基酸后，蛋氨酸（Met）被证明是最有效的添加剂。添加剂的最适宜条件是4 mM Met和1 g/l酵母提取物的组合，ERG的最大产量达到约1.7 mg/l，这相当于不含Met的基础培养基中产量（0.7 mg/l）的2.4倍。尽管添加Met提高了ERG产量，但菌丝体生长受到显著抑制。此外，对培养基中氨基酸的分析表明，添加Met降低了大多数测试氨基酸的消耗率，这可能是由于菌丝体生长减少所致。考虑到这些结果，我们认为向菌丝体培养基中添加Met是提高经济上重要的蘑菇物种中ERG产量的有效方法。
