Mallory Allison C, Elmayan Taline, Vaucheret Hervé
Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA, 78026 Versailles Cedex, France.
Curr Opin Plant Biol. 2008 Oct;11(5):560-6. doi: 10.1016/j.pbi.2008.06.008. Epub 2008 Aug 6.
MicroRNAs are endogenously produced 21-nt riboregulators that associate with ARGONAUTE (AGO) proteins to direct mRNA cleavage or repress translation of complementary RNAs. In addition to protein-coding gene repression, miRNA-directed regulation of non-protein-coding transcripts can incite production of trans-acting siRNA (tasiRNA) populations that themselves direct mRNA repression. Arabidopsis encodes 10 AGO proteins among which, AGO1, AGO7, and AGO10 have been implicated in miRNA-guided gene repression in vivo. Recent work has shown that AGO proteins discriminate their associated small RNA populations on the basis of size and 5'-terminal nucleotide identity, extending the roles of AGO proteins beyond small RNA action. Our expanding appreciation of miRNA-directed regulation during plant development and stress adaptations has placed miRNAs at the forefront of plant biology.
微小RNA是内源性产生的21个核苷酸的核糖调节因子，它们与AGO（精氨酸）蛋白结合，以指导mRNA切割或抑制互补RNA的翻译。除了抑制蛋白质编码基因外，微小RNA对非蛋白质编码转录本的调控还可引发反式作用小干扰RNA（tasiRNA）群体的产生，这些tasiRNA本身也可指导mRNA的抑制。拟南芥编码10种AGO蛋白，其中AGO1、AGO7和AGO10已被证明在体内参与微小RNA引导的基因抑制。最近的研究表明，AGO蛋白根据大小和5'端核苷酸同一性来区分与其相关的小RNA群体，这将AGO蛋白的作用扩展到了小RNA作用之外。我们对植物发育和应激适应过程中微小RNA介导的调控的认识不断扩展，这使得微小RNA处于植物生物学的前沿。
