Stendahl John C, Wang Ling-Jia, Chow Lesley W, Kaufman Dixon B, Stupp Samuel I
Institute for BioNanotechnology in Medicine, Northwestern University, Chicago, IL, USA.
Transplantation. 2008 Aug 15;86(3):478-81. doi: 10.1097/TP.0b013e3181806d9d.
Recent advances in nanotechnology and molecular self-assembly may provide novel solutions to current cell transplantation deficiencies. Heparin-binding peptide amphiphiles (HBPAs) self-assemble from aqueous media into nanofibers that bind growth factors through interactions with the bioactive polymer heparin. In this report, we demonstrate that delivery of vascular endothelial growth factor and fibroblast growth factor-2 from HBPA scaffolds significantly increases blood vessel density in the mouse omentum over control scaffolds without growth factors (P<0.0005) and significantly enhances islet engraftment. Diabetic recipients transplanted with 250 isologous islets and HBPA scaffolds containing vascular endothelial growth factor/fibroblast growth factor-2 achieved normoglycemia at a higher rate (78%) than control animals receiving identical scaffolds without growth factors (30%; P<0.05) or growth factors alone (20%). These data indicate that the enhanced engraftment can be attributed to specific growth factor effects that were made possible by the delivery mechanism of HBPA nanostructures.
纳米技术和分子自组装领域的最新进展可能为当前细胞移植的不足之处提供新的解决方案。肝素结合肽两亲分子（HBPA）在水性介质中自组装成纳米纤维，通过与生物活性聚合物肝素的相互作用结合生长因子。在本报告中，我们证明，与不含生长因子的对照支架相比，从HBPA支架递送血管内皮生长因子和成纤维细胞生长因子-2可显著增加小鼠大网膜中的血管密度（P<0.0005），并显著增强胰岛植入。移植了250个同种胰岛和含有血管内皮生长因子/成纤维细胞生长因子-2的HBPA支架的糖尿病受体实现正常血糖水平的比例（78%）高于接受不含生长因子的相同支架的对照动物（30%；P<0.05）或单独接受生长因子的对照动物（20%）。这些数据表明，植入的增强可归因于HBPA纳米结构的递送机制所实现的特定生长因子效应。
