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一种使用水凝胶贴片的用于病毒和细菌感染的经皮接种系统。

A transcutaneous vaccination system using a hydrogel patch for viral and bacterial infection.

作者信息

Ishii Yumiko, Nakae Tomoko, Sakamoto Fumiko, Matsuo Kazuhiko, Matsuo Keisuke, Quan Ying-Shu, Kamiyama Fumio, Fujita Takuya, Yamamoto Akira, Nakagawa Shinsaku, Okada Naoki

机构信息

Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.

出版信息

J Control Release. 2008 Oct 21;131(2):113-20. doi: 10.1016/j.jconrel.2008.07.025. Epub 2008 Jul 23.

DOI:10.1016/j.jconrel.2008.07.025
PMID:18700159
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7114651/
Abstract

One of the most important anthropic missions is preventing the global spread of infectious diseases. Vaccination is the only available preventive treatment for infectious diseases, but the availability of vaccines in developing countries is not adequate. We report a simple, easy-to-use, noninvasive hydrogel patch transcutaneous vaccination system. Antigen (Ag)-specific IgG production was induced by applying an Ag-immersed patch to non-pretreated mouse auricle or hairless rat back skin. Immunofluorescence histochemical analysis revealed that Langerhans cells resident in the epidermal layer captured the antigenic proteins delivered by the hydrogel patch, which promoted the penetration of antigenic proteins through the stratum corneum, and that Ag-capturing Langerhans cells migrated into draining lymph nodes. Humoral immunity elicited by our transcutaneous vaccination system demonstrated neutralizing activity in both adenoviral infection and passive-challenge tetanus toxin experiments. The use of this hydrogel patch transcutaneous vaccination system will facilitate the global distribution of effective and convenient vaccines.

摘要

最重要的人类使命之一是防止传染病在全球传播。疫苗接种是唯一可用的传染病预防治疗方法，但发展中国家的疫苗供应不足。我们报告了一种简单、易用、非侵入性的水凝胶贴片经皮疫苗接种系统。通过将浸有抗原（Ag）的贴片应用于未经预处理的小鼠耳廓或无毛大鼠背部皮肤，可诱导产生抗原特异性IgG。免疫荧光组织化学分析显示，驻留在表皮层的朗格汉斯细胞捕获了水凝胶贴片递送的抗原蛋白，这促进了抗原蛋白穿过角质层的渗透，并且捕获抗原的朗格汉斯细胞迁移到引流淋巴结。我们的经皮疫苗接种系统引发的体液免疫在腺病毒感染和被动攻击破伤风毒素实验中均表现出中和活性。这种水凝胶贴片经皮疫苗接种系统的使用将有助于有效且便捷的疫苗在全球范围内的分发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/760aedb8ed6f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/2267835a7f65/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/4fb7216a310c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/be8956166390/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/c37785efc5c7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/760aedb8ed6f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/2267835a7f65/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/4fb7216a310c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/be8956166390/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/c37785efc5c7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5924/7114651/760aedb8ed6f/gr5.jpg

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