Kaku Masato, Motokawa Masahide, Tohma Yuiko, Tsuka Natsumi, Koseki Hiroyuki, Sunagawa Hiroko, Arturo Marquez Hernandes Rene, Ohtani Junji, Fujita Tadashi, Kawata Toshitsugu, Tanne Kazuo
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
Biomed Res. 2008 Aug;29(4):181-7. doi: 10.2220/biomedres.29.181.
It has been reported that both vascular endothelial growth factor (VEGF) and macrophage colonystimulating factor (M-CSF) can induce osteoclast recruitment. Thus, VEGF and M-CSF are considered to be closely involved in the bone remodeling process. The purpose of this study was to evaluate changes in VEGF and M-CSF expression during orthodontic treatment. The expression of VEGF and M-CSF mRNA in osteoblasts and fibroblasts was detected by in situ hybridization during experimental tooth movement in mice. Furthermore, the canine retraction side and the control side of orthodontic patients were compared, revealing a statistically significant increase in both VEGF and M-CSF concentrations in gingival crevicular fluid. These results suggest that orthodontic tooth movement causes an increase in VEGF and M-CSF levels. These factors may induce bone remodeling via osteoclastic bone resorption.
据报道，血管内皮生长因子（VEGF）和巨噬细胞集落刺激因子（M-CSF）均可诱导破骨细胞募集。因此，VEGF和M-CSF被认为与骨重塑过程密切相关。本研究的目的是评估正畸治疗期间VEGF和M-CSF表达的变化。通过原位杂交检测小鼠实验性牙齿移动过程中成骨细胞和成纤维细胞中VEGF和M-CSF mRNA的表达。此外，比较了正畸患者的犬牙后缩侧和对照侧，发现龈沟液中VEGF和M-CSF浓度均有统计学意义的增加。这些结果表明，正畸牙齿移动会导致VEGF和M-CSF水平升高。这些因子可能通过破骨细胞骨吸收诱导骨重塑。
