Wise Joel K, Yarin Alexander L, Megaridis Constantine M, Cho Michael
Department of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, USA.
Tissue Eng Part A. 2009 Apr;15(4):913-21. doi: 10.1089/ten.tea.2008.0109.
Cell differentiation, adhesion, and orientation are known to influence the functionality of both natural and engineered tissues, such as articular cartilage. Several attempts have been devised to regulate these important cellular behaviors, including application of inexpensive but efficient electrospinning that can produce patterned extracellular matrix (ECM) features. Electrospun and oriented polycaprolactone (PCL) scaffolds (500 or 3000 nm fiber diameter) were created, and human mesenchymal stem cells (hMSCs) were cultured on these scaffolds. Cell viability, morphology, and orientation on the fibrous scaffolds were quantitatively determined as a function of time. While the fiber-guided initial cell orientation was maintained even after 5 weeks, cells cultured in the chondrogenic media proliferated and differentiated into the chondrogenic lineage, suggesting that cell orientation is controlled by the physical cues and minimally influenced by the soluble factors. Based on assessment by the chondrogenic markers, use of the nanofibrous scaffold (500 nm) appears to enhance the chondrogenic differentiation. These findings indicate that hMSCs seeded on a controllable PCL scaffold may lead to an alternate methodology to mimic the cell and ECM organization that is found, for example, in the superficial zone of articular cartilage.
细胞分化、黏附和定向已知会影响天然组织和工程组织（如关节软骨）的功能。人们已经设计了几种方法来调节这些重要的细胞行为，包括应用廉价但高效的静电纺丝技术，该技术可以产生有图案的细胞外基质（ECM）特征。制备了静电纺丝且定向的聚己内酯（PCL）支架（纤维直径为500或3000纳米），并将人间充质干细胞（hMSCs）培养在这些支架上。定量测定了纤维支架上细胞活力、形态和定向随时间的变化。尽管即使在5周后纤维引导的初始细胞定向仍得以维持，但在软骨形成培养基中培养的细胞增殖并分化为软骨形成谱系，这表明细胞定向受物理线索控制，且受可溶性因子的影响最小。基于软骨形成标志物的评估，使用纳米纤维支架（500纳米）似乎能增强软骨形成分化。这些发现表明，接种在可控PCL支架上的hMSCs可能会带来一种替代方法，以模拟例如在关节软骨表层区域发现的细胞和ECM组织。
