Li Xiaolong, Wu Qingmin, Zhang Xiaoxue, Li Chong, Zhang Deyin, Li Guoze, Zhang Yukun, Zhao Yuan, Shi Zhaoguo, Wang Weimin, Li Fadi
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
College of Veterinary Medicine, China Agricultural University, Beijing, China.
Front Genet. 2021 Jul 8;12:653927. doi: 10.3389/fgene.2021.653927. eCollection 2021.
Brucellosis is a zoonotic disease and a major public health problem. However, the genetic mechanism of brucellosis in sheep remains unclear. In this study, serum samples were collected from 6,358 sheep from the F2 population (Dorper sheep ♂ × Hu sheep ♀), and antibody levels were continuously measured at 14 days and 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 months after administration of brucellosis vaccine. Finally, 19 brucellosis-resistant group (BRG) sheep and 22 brucellosis-susceptible group sheep (BSG) were screened for whole-genome sequencing. Using the fixation index, Fisher's exact test, and chi-square test, a total of 205 candidate SNP sites were identified. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis suggested that 138 candidate genes were significantly enriched in adherens junction (, , and ), cell adhesion molecules (, , , and ), salivary secretion (, , and ), and hippo signaling pathway (, , and ). These findings provide valuable molecular markers for brucellosis resistance breeding in sheep and novel insights into the genetic mechanism of brucellosis resistance.
布鲁氏菌病是一种人畜共患病，也是一个主要的公共卫生问题。然而，绵羊布鲁氏菌病的遗传机制仍不清楚。在本研究中，从6358只F2代绵羊群体（杜泊羊♂×湖羊♀）中采集血清样本，并在接种布鲁氏菌病疫苗后的14天以及1、2、3、4、5、6、7、8、9和10个月连续测量抗体水平。最后，筛选出19只布鲁氏菌病抗性组（BRG）绵羊和22只布鲁氏菌病易感组绵羊（BSG）进行全基因组测序。通过固定指数、Fisher精确检验和卡方检验，共鉴定出205个候选单核苷酸多态性（SNP）位点。京都基因与基因组百科全书（KEGG）通路富集分析表明，138个候选基因在黏着连接（、和）、细胞黏附分子（、、和）、唾液分泌（、和）以及河马信号通路（、和）中显著富集。这些发现为绵羊布鲁氏菌病抗性育种提供了有价值的分子标记，并为布鲁氏菌病抗性的遗传机制提供了新的见解。
