FIH-1的自羟基化作用：一种依赖于亚铁离子和α-酮戊二酸的人类低氧传感器。
Auto-hydroxylation of FIH-1: an Fe(ii), alpha-ketoglutarate-dependent human hypoxia sensor.
作者信息
Chen Yuan-Han, Comeaux Lindsay M, Eyles Stephen J, Knapp Michael J
机构信息
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
出版信息
Chem Commun (Camb). 2008 Oct 21(39):4768-70. doi: 10.1039/b809099h. Epub 2008 Aug 11.
Abstract
HIF-asparaginyl hydroxylase (FIH-1) normally couples O(2)-activation to hydroxylation of Asn(803) on the alpha-subunit of the hypoxia-inducible factor (HIFalpha), a key step in pO(2) sensing; in the absence of HIFalpha, O(2)-activation becomes uncoupled, leading to self-hydroxylation at Trp(296) and a purple Fe(iii)-O-Trp chromophore-this alternative reactivity may affect human hypoxia sensing.
摘要
缺氧诱导因子天冬酰胺羟化酶（FIH-1）通常将氧激活与缺氧诱导因子（HIFα）α亚基上Asn（803）的羟基化偶联起来，这是氧分压（pO₂）传感中的关键步骤；在缺乏HIFα的情况下，氧激活会解偶联，导致Trp（296）处的自羟基化以及紫色的Fe（III）-O-Trp发色团——这种替代反应性可能会影响人类的缺氧传感。
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