Wang Hong-Yan, Pan Ya-Ping, Teng Di, Zhao Jian, Lin Li
Department of Periodontology, School of Stomatology, China Medical University, Shenyang 110002, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2008 Apr;43(4):236-9.
To investigate the relationship between chronic periodontitis and the genetic polymorphisms of vitamin D receptor gene and estrogen receptor gene.
Clinical parameters including probing depth, clinical attachment loss, sulcus bleeding index and tooth movement were measured by fluoride probe. Genomic DNA from peripheral venous blood was extracted with saturant sodium chloride, and PCR-restriction fragment length polymorphism was applied to examine the Apa I, Bsm I, Taq I polymorphisms of the vitamin D receptor genes and the Xba I and Pvu II polymorphisms of the estrogen receptor genes. The results were analyzed by Z-score test and mean square analysis.
Forty-three point four percent of chronic periodontitis patients took vitamin D receptor BB genotype, the rate in healthy controls was 30.0%. 39.6% of chronic periodontitis patients took estrogen receptor XX genotype, the rate in healthy controls was 20.0%. The people who took BBXX genotype had the worst periodontal conditions among all chronic periodontitis patients.
Vitamin D receptor allele B and estrogen receptor allele X are susceptible alleles for chronic periodontitis. The synergistic effects of the two receptor susceptible alleles may promote chronic periodontitis.
探讨慢性牙周炎与维生素D受体基因及雌激素受体基因多态性之间的关系。
用氟探针测量临床参数，包括探诊深度、临床附着丧失、龈沟出血指数和牙齿移动度。用饱和氯化钠从外周静脉血中提取基因组DNA，采用聚合酶链反应-限制性片段长度多态性技术检测维生素D受体基因的Apa I、Bsm I、Taq I多态性以及雌激素受体基因的Xba I和Pvu II多态性。结果采用Z评分检验和均方分析。
43.4%的慢性牙周炎患者为维生素D受体BB基因型，健康对照组为30.0%。39.6%的慢性牙周炎患者为雌激素受体XX基因型，健康对照组为20.0%。在所有慢性牙周炎患者中，携带BBXX基因型的人牙周状况最差。
维生素D受体基因B等位基因和雌激素受体基因X等位基因是慢性牙周炎的易感等位基因。两种受体易感等位基因的协同作用可能促进慢性牙周炎。
