Schmid-Schwap Martina, Franz Alexander, König Franz, Bristela Margit, Lucas Trevor, Piehslinger Eva, Watts David C, Schedle Andreas
Department of Prosthodontics, Bernhard Gottlieb University Clinic of Dentistry, Medical University of Vienna, Austria.
Dent Mater. 2009 Mar;25(3):360-8. doi: 10.1016/j.dental.2008.08.002. Epub 2008 Oct 11.
Assessment of dental material biocompatibility is gaining increasing importance for both patients and dentists. Dental cements may be in contact with oral soft tissues for prolonged periods of time and play an important role in prosthetic rehabilitation. The aim of the present study was to evaluate eight dental cements using a standardized L929-fibroblast cell culture test.
For each material, fresh specimens (added to the cultures immediately after preparation) and specimens preincubated for 7 days in cell culture medium were prepared according to the manufacturers' recommendations. After exposure to test specimens, cell numbers were compared to glass controls. The main outcome was a two-sided 95% confidence interval for the mean value of the standardized cell number for each substance investigated.
Fresh specimens of all tested cements showed significant cytotoxicity, which diminished after 7 days preincubation. Cytotoxicity of fresh adhesive and self-adhesive resin cements was lower when specimens were dual-cured compared to self-cured. A rank order of cytotoxicity was established based on mean values: Nexus 2 (dual-cured) showed least cytotoxicity, followed by Variolink II (dual-cured), Nexus 2 (self-cured), Harvard, RelyxUnicem (dual-cured), Panavia 21, Fujicem, Durelon, Variolink II (self-cured), RelyxUnicem (self-cured), Maxcem (dual-cured) and Maxcem (self-cured). When bondings were added to Nexus 2 or Variolink II specimens, a slight increase in cytotoxicity was observed.
Adhesive resin cements showed less cytotoxicity than self-adhesive and chemically setting cements. Bonding only slightly influenced cytotoxicity of the adhesive resin cements. Dual-cured specimens of adhesive and self-adhesive resin cements showed significantly less toxicity than self-cured specimens.
评估牙科材料的生物相容性对患者和牙医而言都变得越来越重要。牙科粘固剂可能会与口腔软组织长时间接触，并在修复性康复中发挥重要作用。本研究的目的是使用标准化的L929成纤维细胞培养试验评估八种牙科粘固剂。
对于每种材料，根据制造商的建议制备新鲜标本（制备后立即添加到培养物中）和在细胞培养基中预孵育7天的标本。暴露于测试标本后，将细胞数量与玻璃对照进行比较。主要结果是每种研究物质标准化细胞数平均值的双侧95%置信区间。
所有测试粘固剂的新鲜标本均显示出明显的细胞毒性，在预孵育7天后细胞毒性降低。与自固化相比，双固化标本时，新鲜的粘结性和自粘结性树脂粘固剂的细胞毒性较低。根据平均值确定了细胞毒性的等级顺序：Nexus 2（双固化）显示出最小的细胞毒性，其次是Variolink II（双固化）、Nexus 2（自固化）、Harvard、RelyxUnicem（双固化）、Panavia 21、Fujicem、Durelon、Variolink II（自固化）、RelyxUnicem（自固化）、Maxcem（双固化）和Maxcem（自固化）。当向Nexus 2或Variolink II标本中添加粘结剂时，观察到细胞毒性略有增加。
粘结性树脂粘固剂的细胞毒性低于自粘结性和化学固化粘固剂。粘结仅对粘结性树脂粘固剂的细胞毒性有轻微影响。粘结性和自粘结性树脂粘固剂的双固化标本显示出的毒性明显低于自固化标本。
