Ott M, Bender L, Marre R, Hacker J
Institut für Genetik und Mikrobiologie, University of Wurzburg, Germany.
J Clin Microbiol. 1991 Apr;29(4):813-5. doi: 10.1128/jcm.29.4.813-815.1991.
Ten Legionella pneumophila strains isolated from different sources were analyzed according to their restriction fragment patterns obtained by cleavage of genomic DNA with NotI and SfiI and separation by pulsed field electrophoresis. Three L. pneumophila isolates from a nosocomial outbreak in Lübeck (Germany) and three other L. pneumophila strains independently isolated from a water tap located in the care unit where the patients were hospitalized exhibited identical restriction fragment profiles. Therefore, we concluded that these environmental specimens were the source of the Legionnaires disease. Another two isolates from patients and two strains from the environment, all unrelated to the outbreak described, showed different cleavage patterns.
对从不同来源分离出的10株嗜肺军团菌菌株，根据用NotI和SfiI酶切基因组DNA并通过脉冲场电泳分离后获得的限制性片段模式进行分析。从吕贝克（德国）一家医院爆发疫情中分离出的3株嗜肺军团菌菌株，以及从患者住院所在护理单元的一个水龙头独立分离出的另外3株嗜肺军团菌菌株，呈现出相同的限制性片段图谱。因此，我们得出结论，这些环境标本是军团病的源头。另外两株来自患者的分离株和两株来自环境的菌株，均与所描述的疫情无关，显示出不同的酶切模式。
