van Ketel R J, de Wever B
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
J Clin Microbiol. 1989 May;27(5):1105-7. doi: 10.1128/jcm.27.5.1105-1107.1989.
Legionella pneumophila strains isolated from six patients, three air-conditioning- and cooling tower-derived strains, and three hot water supply-derived strains were analyzed by three genetic typing methods. The results of the whole-cell DNA restriction endonuclease analysis and the restriction patterns based on genes coding for rRNA correlated with each other and demonstrated that the patient isolates were indistinguishable from the air-conditioning- and cooling tower-derived isolates but differed markedly from the hot water supply-derived isolates. The patient and air-conditioning- and cooling tower-derived strains contained plasmids of the same molecular weight; the hot water supply-derived strains were plasmidless. These results indicated that the cooling tower or the air-conditioning system was the environmental source for the examined cluster of Legionnaires disease strains.
对从6名患者中分离出的嗜肺军团菌菌株、3株源自空调和冷却塔的菌株以及3株源自热水供应系统的菌株，采用三种基因分型方法进行了分析。全细胞DNA限制性内切酶分析结果和基于rRNA编码基因的限制性图谱相互关联，表明从患者中分离出的菌株与源自空调和冷却塔的菌株无法区分，但与源自热水供应系统的菌株明显不同。患者以及源自空调和冷却塔的菌株含有分子量相同的质粒；源自热水供应系统的菌株无质粒。这些结果表明，冷却塔或空调系统是所检测的军团病菌株群的环境来源。
