Comstock L E, Thomas D D
Department of Microbiology and Immunology, Wake Forest University Medical Center, Winston-Salem, North Carolina 27103.
Microb Pathog. 1991 Feb;10(2):137-48. doi: 10.1016/0882-4010(91)90074-k.
Borrelia burgdorferi can adhere to cultured endothelial cells and penetrate through cell monolayers by passing through intercellular tight junctions and through the host cell cytoplasm. Borrelia burgdorferi strains which were isolated from different sources and areas of the U.S. all demonstrated similar invasive capabilities. Bacterial penetration from the apical to the basal surface of the monolayer was 20 times more efficient than from the basal to the apical surface. Borreliae which were non-viable as a result of either heat treatment or ultraviolet (UV) irradiation showed reduced association with the endothelial cell monolayer and loss of invasive capabilities. Borreliae were able to invade when protein synthesis was inhibited with streptomycin or chloramphenicol. When assays were conducted at 4 degrees C, bacterial penetration of the monolayer was completely inhibited. Treatment of borreliae with proteases affecting outer surface proteins greatly reduced cell association and bacterial invasion.
伯氏疏螺旋体能够黏附于培养的内皮细胞，并通过细胞间紧密连接和宿主细胞质穿透细胞单层。从美国不同来源和地区分离出的伯氏疏螺旋体菌株均表现出相似的侵袭能力。细菌从单层细胞的顶端向基底表面的穿透效率比从基底向顶端表面高20倍。由于热处理或紫外线（UV）照射而失去活力的疏螺旋体与内皮细胞单层的结合减少，侵袭能力丧失。当用链霉素或氯霉素抑制蛋白质合成时，疏螺旋体仍能够侵袭。当在4℃进行试验时，细菌对单层细胞的穿透被完全抑制。用影响外表面蛋白的蛋白酶处理疏螺旋体，可大大减少细胞结合和细菌侵袭。
