Piironen Vieno, Edelmann Minnamari, Kariluoto Susanna, Bedo Zoltan
Department of Applied Chemistry and Microbiology, P.O. Box 27, University of Helsinki, FIN-00014 University of Helsinki, Finland.
J Agric Food Chem. 2008 Nov 12;56(21):9726-31. doi: 10.1021/jf801066j. Epub 2008 Oct 15.
As part of the diversity screen of the HEALTHGRAIN project, the total folate contents of bread wheat (130 winter and 20 spring wheat genotypes), durum wheat (10 genotypes), earlier cultivated diploid einkorn and tetraploid emmer wheat (5 genotypes of each), and spelt (5 genotypes), grown in the same location in a controlled manner, were determined by a microbiological assay. The total folate contents ranged from 364 to 774 ng/g of dm in winter wheat and from 323 to 741 ng/g of dm in spring wheat, thus showing a marked variation. The highest mean for total folate content was measured in the durum wheat genotypes, whereas the earlier cultivated diploid and tetraploid wheat genotypes and spelt were shown to possess comparable or even higher folate contents than bread wheat. HPLC analysis of selected genotypes showed that 5-formyltetrahydrofolate was the major vitamer. The data provide a basis for breeding wheat genotypes with improved folate content.
作为HEALTHGRAIN项目多样性筛选的一部分，采用微生物分析法测定了在同一地点以可控方式种植的面包小麦（130个冬小麦基因型和20个春小麦基因型）、硬粒小麦（10个基因型）、早期种植的二倍体单粒小麦和四倍体二粒小麦（各5个基因型）以及斯佩尔特小麦（5个基因型）的总叶酸含量。冬小麦的总叶酸含量在364至774 ng/g干物质之间，春小麦的总叶酸含量在323至741 ng/g干物质之间，呈现出显著差异。硬粒小麦基因型的总叶酸含量均值最高，而早期种植的二倍体和四倍体小麦基因型以及斯佩尔特小麦的叶酸含量与面包小麦相当，甚至更高。对选定基因型的HPLC分析表明，5-甲酰基四氢叶酸是主要的维生素形式。这些数据为培育叶酸含量更高的小麦基因型提供了依据。
