Li Xue-Mei, Zhan Zhi-Ming, Ju Heng-Qiang, Zhang Shu-Sheng
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, People's Republic of China.
Oligonucleotides. 2008 Dec;18(4):321-7. doi: 10.1089/oli.2008.0143.
A novel label-free electrochemical DNA biosensor based on 4,4'-diaminoazobenzene (4,4'-DAAB) and multiwalled carbon nanotube (MWNT)-modified glassy carbon electrode (GCE) for short DNA sequences related to the hepatitis B virus (HBV) hybridization detection was presented. Differential pulse voltammetry (DPV) was used to investigate hybridization event. The decrease in the peak current of 4,4'-DAAB was observed on hybridization of probe with the target. This electrochemical approach was sequence specific as indicated by the control experiments, in which no peak current change was observed when a noncomplementary DNA sequence was used. Numerous factors affecting the target hybridization were optimized to maximize the sensitivity. Under optimal conditions, this sensor showed a good calibration range between 7.94 x 10(-8) M and 1.58 x 10(-6) M, with HBV DNA sequence detection limit of 1.1 x 10(-8) M.
提出了一种基于4,4'-二氨基偶氮苯（4,4'-DAAB）和多壁碳纳米管（MWNT）修饰玻碳电极（GCE）的新型无标记电化学DNA生物传感器，用于检测与乙型肝炎病毒（HBV）相关的短DNA序列的杂交。采用差分脉冲伏安法（DPV）研究杂交事件。在探针与靶标杂交时观察到4,4'-DAAB的峰电流下降。对照实验表明，这种电化学方法具有序列特异性，当使用非互补DNA序列时未观察到峰电流变化。对影响靶标杂交的众多因素进行了优化，以最大限度地提高灵敏度。在最佳条件下，该传感器在7.94×10^(-8) M至1.58×10^(-6) M之间显示出良好的校准范围，HBV DNA序列检测限为1.1×10^(-8) M。
