For reprints and all correspondence: Bo-Ying Chen, Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Fudan University, No.138, Yi Xue Yuan Road, Shanghai 200032, Shanghai, China. +86-21-54237693;
Evid Based Complement Alternat Med. 2009 Mar;6(1):51-6. doi: 10.1093/ecam/nem062. Epub 2007 Oct 27.
We explored the neurochemical mechanism of electroacupuncture's (EA) protective effect on brain function in focal cerebral ischemia rats, using cerebral ischemia/reperfusion rats established by the middle cerebral artery occlusion (MCAO) method. Adult male Sprague-Dawley rats were randomly divided into four groups: Sham, Sham+EA, MCAO and MCAO+EA. The rats in Sham+EA and MCAO+EA were accepted EA treatment at 'GV26' and 'GV20' acupoints for 30 min. Electric stimulation was produced by a G-6805 generator and neurological deficit scores were recorded. Mitochondria respiratory function and the activities of respiratory enzymes were measured by a computer-aided Clark oxygen electrode system. Results showed that EA treatment might reduce the neurological deficit score, and significantly improve respiratory control ratio (RCR), the index of mitochondrial respiratory function, and increase the activities of succinic dehydrogenase, NADH dehydrogenase and cytochrome C oxidase in the MCAO rats. Results suggest that EA might markedly decrease the neurological deficit score, promote the activities of respiratory enzymes and reduce the generation of reactive oxygen species (ROS), resulting in improvement of respiratory chain function and anti-oxidative capability of brain tissues in the infarct penumbra zone. This be a mechanism of EA's anti-injury effect on brain function in MCAO rats.
我们采用大脑中动脉阻塞（MCAO）方法建立脑缺血/再灌注大鼠模型，探讨电针对脑缺血后大鼠脑功能保护作用的神经化学机制。成年雄性 Sprague-Dawley 大鼠随机分为四组：假手术组、假手术+电针组、MCAO 组和 MCAO+电针组。假手术+电针组和 MCAO+电针组大鼠接受电针刺激“GV26”和“GV20”穴位 30 分钟。电刺激由 G-6805 发生器产生，并记录神经功能缺损评分。通过计算机辅助 Clark 氧电极系统测量线粒体呼吸功能和呼吸酶的活性。结果表明，电针治疗可能降低神经功能缺损评分，显著改善呼吸控制比（RCR），即线粒体呼吸功能指标，并增加 MCAO 大鼠中琥珀酸脱氢酶、NADH 脱氢酶和细胞色素 C 氧化酶的活性。结果提示，电针可能显著降低神经功能缺损评分，促进呼吸酶的活性，减少活性氧（ROS）的产生，从而改善梗死半影区的呼吸链功能和脑组织的抗氧化能力。这是电针对 MCAO 大鼠脑功能损伤保护作用的机制之一。
