Kiyohara H, Takemoto N, Komatsu Y, Kawamura H, Hosoya E, Yamada H
Oriental Medicine Research Center, Kitasato Institute, Tokyo Japan.
Planta Med. 1991 Jun;57(3):254-9. doi: 10.1055/s-2006-960085.
The acidic polysaccharide fraction (F-5-2) from "Juzen-Taiho-To" (TJ-48), a Kampo (Japanese herbal) medicine prepared by decocting a prescription of 10 kinds of herbs, has potent mitogenic activity. In order to clarify the mitogenic activity, F-5-2 was fractionated by amon-exchange chromatography, and 14 acidic polysaccharide fractions were obtained. Mitogenic activities of these polysaccharides were increasing with their molecular masses and affinities to the anion-exchange column. Methylation analysis and endo-alpha-(1----4)-polygalacturonase digestion showed these polysaccharide fractions to be pectic polysaccharides, many of which consisted mainly of polygalacturonan regions in addition to small amounts of "ramified" regions. Gel filtration showed that the molecular masses of the "ramified" regions of the polysaccharides were similar. High molecular mass polygalacturonic acid showed a weak mitogenic activity. The mitogenic (M-1 and M-2) and non-mitogenic (M-3) polysaccharides from the most active polysaccharide fraction (F-5-2IIh) were further purified by gel filtration. M-2 and M-3 were also shown to be pectic polysaccharides, and the neutral glycosidic linkages in M-2 and M-3 were different from each other. Endopolygalacturonase digestion markedly decreased the mitogenic activity of M-2, however, the "ramified" region of M-2 still showed a weak activity.
“十全大补汤”（TJ - 48）是一种通过煎煮10种草药的配方制成的日本汉方药物，其酸性多糖组分（F - 5 - 2）具有强大的促有丝分裂活性。为了阐明这种促有丝分裂活性，F - 5 - 2通过阴离子交换色谱进行分离，得到了14个酸性多糖组分。这些多糖的促有丝分裂活性随着它们的分子量以及与阴离子交换柱的亲和力而增加。甲基化分析和内切α -（1→4）-聚半乳糖醛酸酶消化表明这些多糖组分是果胶多糖，其中许多除了少量“分支”区域外，主要由聚半乳糖醛酸区域组成。凝胶过滤显示多糖“分支”区域的分子量相似。高分子量的聚半乳糖醛酸显示出较弱的促有丝分裂活性。来自活性最强的多糖组分（F - 5 - 2IIh）的促有丝分裂（M - 1和M - 2）和无促有丝分裂活性（M - 3）的多糖通过凝胶过滤进一步纯化。M - 2和M - 3也被证明是果胶多糖，并且M - 2和M - 3中的中性糖苷键彼此不同。内切聚半乳糖醛酸酶消化显著降低了M - 2的促有丝分裂活性，然而，M - 2的“分支”区域仍显示出较弱的活性。
