身份检测：细胞系交叉污染的鉴定。
Identity tests: determination of cell line cross-contamination.
机构信息
Stem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves, Avenida de las Fuerzas Armadas No. 2, 18014, Granada, Spain,
出版信息
Cytotechnology. 2006 Jun;51(2):45-50. doi: 10.1007/s10616-006-9013-8. Epub 2006 Aug 3.
Abstract
Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses.
摘要
细胞系交叉污染是由于连续细胞系培养而产生的现象。据估计，大约有 20%的细胞系被错误鉴定，因此有必要进行质量控制测试以检测这个问题。自从发现细胞系交叉污染以来，已经应用了不同的方法，例如同工酶分析用于物种间的交叉污染；HLA 分型，以及使用短串联重复和可变串联重复的 DNA 指纹分析用于种内的交叉污染。从这个意义上说，细胞库是负责保证细胞系真实性的组织，以便未来的研究和临床应用。
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