抗人参主要成分人参皂苷 Rb1 的单克隆抗体的制备及其特性鉴定。
Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization.
机构信息
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812, Japan.
出版信息
Cytotechnology. 1999 Mar;29(2):115-20. doi: 10.1023/A:1008068718392.
Abstract
The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.
摘要
通过基质辅助激光解吸/电离质谱法确定抗原缀合物中半抗原和牛血清白蛋白的比例。用人参皂苷 Rb1-牛血清白蛋白缀合物免疫的脾细胞与 HAT 敏感的小鼠骨髓瘤细胞系 P3-X63-Ag8-653 融合，产生了一株分泌针对人参皂苷 Rb1 的单克隆抗体的杂交瘤。与人参皂苷 Rc 和人参皂苷 Rd 出现很小的交叉反应。该测定法的全测量范围从 20ngml-1 到 400ngml-1 的人参皂苷 Rb1。
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