Department of Applied Life Science, Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Cytotechnology. 2015 Aug;67(4):671-80. doi: 10.1007/s10616-014-9758-4. Epub 2014 Jul 2.
Pterostilbene, a methoxylated analogue of resveratrol, is a natural compound primarily found in blueberries and several types of grapes. However, little is known about the effect of pterostilbene on the proliferation of hepatoma cells and its modes of actions. This study was undertaken to characterize its ability to suppress the proliferation of hepatoma AH109A cells and the possible mechanism(s) involved. Pterostilbene showed a significant and dose-dependent effect on the anti-proliferative activity against AH109A cells. Pterostilbene exerted little or no effect on the proliferation of rat L6 myoblasts and rat skin fibroblasts. Pterostilbene-loaded rat sera could significantly inhibit the proliferation of AH109A cells, which suggests that pterostilbene could be absorbed through gastrointestinal tract and retain its anti-proliferative activity. Pterostilbene arrested the cell cycle of AH109A cells at G0/G1 phase and reduced the protein expression of cyclin-dependent kinase 4 and cyclin-dependent kinase 6 dose-dependently. We also found that pterostilbene could significantly increase the intracellular peroxide level of AH109A cells, which may be involved in its anti-proliferative activity.
紫檀芪是白藜芦醇的甲氧基类似物，是一种天然化合物，主要存在于蓝莓和几种葡萄中。然而，人们对紫檀芪对肝癌细胞增殖的影响及其作用方式知之甚少。本研究旨在研究其抑制肝癌 AH109A 细胞增殖的能力及其可能的作用机制。紫檀芪对 AH109A 细胞的抗增殖活性具有显著的剂量依赖性作用。紫檀芪对大鼠 L6 成肌细胞和大鼠皮肤成纤维细胞的增殖几乎没有影响或没有影响。负载紫檀芪的大鼠血清可显著抑制 AH109A 细胞的增殖，这表明紫檀芪可通过胃肠道吸收并保持其抗增殖活性。紫檀芪将 AH109A 细胞的细胞周期阻滞在 G0/G1 期，并呈剂量依赖性降低细胞周期蛋白依赖性激酶 4 和细胞周期蛋白依赖性激酶 6 的蛋白表达。我们还发现紫檀芪可显著增加 AH109A 细胞内过氧化物水平，这可能与其抗增殖活性有关。
