Muscle Biology Laboratory, Department of Animal Sciences , Washington State University, Pullman, WA, 99164, USA.
Cytotechnology. 2004 Sep;46(1):49-56. doi: 10.1007/s10616-004-3903-4. Epub 2005 Jun 16.
Bovine adipofibroblasts, 3T3-L1 cells, L-6 myogenic cells, and sheep satellite cells were allowed to proliferate for 48 h. Oil red-O (ORO) was dissolved in three different solvents isopropanol, propylene glycol and triethyl phosphate. At 48 h, the proliferative cultures were stained with the three stains. ORO stain prepared in both propylene glycol and triethyl phosphate resulted in bright red droplets appearing in all cultures, whereas ORO dissolved in isopropanol was not taken up by any of the cells. These data suggest that certain preparations of ORO may stain cells in non-adipogenic lineages as well as undifferentiated pre-adipocytes. Caution must be exercised when choosing solvents for ORO in differentiation studies using cells of the fat/adipose lineage.
牛脂肪成纤维细胞、3T3-L1 细胞、L-6 成肌细胞和绵羊卫星细胞被允许增殖 48 小时。油红 O（ORO）溶解在三种不同的溶剂异丙醇、丙二醇和三乙基磷酸酯中。在 48 小时时，用三种染色剂对增殖培养物进行染色。在所有培养物中，用丙二醇和三乙基磷酸酯制备的 ORO 染色剂都产生了明显的红色液滴，而用异丙醇溶解的 ORO 则没有被任何细胞吸收。这些数据表明，某些 ORO 制剂可能会对非脂肪生成谱系以及未分化的前脂肪细胞染色。在使用脂肪/脂肪谱系的细胞进行分化研究时，选择 ORO 的溶剂时必须谨慎。
