Adebonojo F O
Yale J Biol Med. 1975 Mar;48(1):9-16.
In an effort to test the adipose hyperplasia theory of obesity in humans, adipose cells, derived from anterior abdominal walls of human infants and children, were grown in synthetic medium (McCoy's 5A Medium) supplemented with 20% fetal calf serum. Adipose cells which became delipidinized in culture were found to be capable of division and the rate and number of cell divisions was age dependent. Cells of infants under 1 yr of age and cells derived from early adolescent children divided to varying degrees in culture. Adipose cells from children aged 1-10 yr showed no cell division. Cell division was never observed in a lipid-laden adipocyte. Measurements of cell diameter showed that after the first year of life, cell size increased progressively with age. During the first year adipose cell size appeared to reflect the rapid hyperplasia of the first 3 mo, reaching smallest size at 3-12 mo but increasing thereafter.
为了验证人类肥胖的脂肪增生理论，从人类婴幼儿和儿童的前腹壁获取脂肪细胞，在添加20%胎牛血清的合成培养基（ McCoy's 5A培养基）中培养。发现在培养中脱脂的脂肪细胞能够分裂，且细胞分裂的速率和数量与年龄有关。1岁以下婴儿的细胞以及来自青春期早期儿童的细胞在培养中会有不同程度的分裂。1 - 10岁儿童的脂肪细胞未显示细胞分裂。在充满脂质的脂肪细胞中从未观察到细胞分裂。细胞直径测量显示，在生命的第一年之后，细胞大小随年龄逐渐增加。在第一年，脂肪细胞大小似乎反映了前3个月的快速增生，在3 - 12个月时达到最小尺寸，但此后会增加。
