假细胞系：问题与解决方案。
False cell lines: The problem and a solution.
机构信息
Prostate Cancer Research Centre, UCL, London, U.K. (E-mail,
出版信息
Cytotechnology. 2002 Jul;39(2):69-74. doi: 10.1023/A:1022908930937.
Abstract
Hundreds of misleading reports are published every year containing data on human cancer cell lines that are derived from some other species, tissue or individual to that claimed. In consequence, millions of dollars provided for cancer research are being spent on the production of misleading data. This review describes how cross-contamination occurs, catalogues the use of false cell lines in leading biomedical journals, and suggests ways to resolve the problem.
摘要
每年都有数百篇误导性报告发表，其中包含的数据来自声称的物种、组织或个体以外的人类癌细胞系。因此，用于癌症研究的数百万美元都被用于产生误导性数据。本文描述了交叉污染是如何发生的，列出了在主要生物医学期刊中使用虚假细胞系的情况，并提出了解决问题的方法。
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本文引用的文献
1
HeLa cells 50 years on: the good, the bad and the ugly.50年过去了，海拉细胞：功过与是非。
Nat Rev Cancer. 2002 Apr;2(4):315-9. doi: 10.1038/nrc775.
2
X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance.X射线修复交叉互补基因I蛋白在喜树碱耐药中起重要作用。
Cancer Res. 2002 Jan 15;62(2):459-65.
3
Cyclooxygenase-2 is up-regulated in proliferative inflammatory atrophy of the prostate, but not in prostate carcinoma.环氧化酶-2在前列腺增生性炎性萎缩中上调，但在前列腺癌中未上调。
Cancer Res. 2001 Dec 15;61(24):8617-23.
4
Cell surface-localized nucleolin is a eukaryotic receptor for the adhesin intimin-gamma of enterohemorrhagic Escherichia coli O157:H7.细胞表面定位的核仁素是肠出血性大肠杆菌O157:H7黏附素intimin-γ的真核受体。
J Biol Chem. 2002 Jan 25;277(4):2876-85. doi: 10.1074/jbc.M110230200. Epub 2001 Nov 9.
5
Responsibility for trust in research.研究中的信任责任。
Nature. 1981 Jan 22;289(5795):211-2. doi: 10.1038/289211b0.
6
7
8
TSU-Pr1 and JCA-1 cells are derivatives of T24 bladder carcinoma cells and are not of prostatic origin.TSU-Pr1细胞和JCA-1细胞是T24膀胱癌细胞的衍生物，并非来源于前列腺。
Cancer Res. 2001 Sep 1;61(17):6340-4.
9
Antiviral activity and structural characteristics of the nonglycosylated central subdomain of human respiratory syncytial virus attachment (G) glycoprotein.人呼吸道合胞病毒附着（G）糖蛋白非糖基化中央亚结构域的抗病毒活性及结构特征
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