Sadeghi Golnar, Khaksar Ali Asghar, Bassiri Jahromi Shahindokht, Amirkhani Aref, Eslamifar Ali, Ajdary Soheila, Katiraee Farzad, Taeb Jaleh, Paskiabi Farnoosh Asghari, Sayyah Mohammad
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran.
J Dermatolog Treat. 2009;20(2):120-3. doi: 10.1080/09546630802449070.
Dermatophytes are one of the main causes of dermal infections. Moreover, there are some opportunistic fungi such as Aspergillus fumigatus (mycelial form) and Candida albicans (yeasty form) that in immunosuppressed patients can cause cutaneous disease.
The possible effect of optical brightener 220 (OB-220) on the growth of fungi has been evaluated in this study.
Isolates were grown on agar plates containing OB-220 in concentration between 0.06 and 11.68 mg ml(-1). MICs of OB-220, ketoconazole and fluconazole were obtained by the agar dilution method. Hyphae and yeasts grown with OB-220 were compared with controls by fluorescence and transmission electron microscopy. The cell cytotoxicity of OB-220 was also assessed.
The MIC(90) of OB-220 was obtained: 1.17-1.46 mg ml(-1) for A. fumigatus, 0.58-1.17 mg ml(-1) for C. albicans and 0.29 mg ml(-1) for Trichophyton tonsurans. Electron microscopy revealed a thickening and blurred contours of the cell wall by OB-220. OB-220 in concentrations up to 11.68 mg ml(-1) posed no mammalian cell toxicity.
OB-220 suppresses the growth of fungi by interfering with the formation of normal chitin.
皮肤癣菌是皮肤感染的主要病因之一。此外，还有一些机会性真菌，如烟曲霉（丝状形态）和白色念珠菌（酵母形态），在免疫抑制患者中可引起皮肤疾病。
本研究评估了荧光增白剂220（OB - 220）对真菌生长的可能影响。
将分离菌株接种在含有浓度为0.06至11.68 mg/ml OB - 220的琼脂平板上。通过琼脂稀释法获得OB - 220、酮康唑和氟康唑的最低抑菌浓度（MIC）。通过荧光显微镜和透射电子显微镜将用OB - 220培养的菌丝和酵母与对照进行比较。还评估了OB - 220的细胞毒性。
获得了OB - 220的MIC90：烟曲霉为1.17 - 1.46 mg/ml，白色念珠菌为0.58 - 1.17 mg/ml，断发毛癣菌为0.29 mg/ml。电子显微镜显示OB - 220使细胞壁增厚且轮廓模糊。浓度高达11.68 mg/ml的OB - 220对哺乳动物细胞无毒性。
OB - 220通过干扰正常几丁质的形成来抑制真菌生长。
