Jaishree V, Badami S, Rupesh Kumar M, Tamizhmani T
Department of Pharmaceutical Chemistry, J.S.S. College of Pharmacy, Ootacamund-643001, Tamil Nadu, India.
Phytomedicine. 2009 Mar;16(2-3):227-32. doi: 10.1016/j.phymed.2008.09.010. Epub 2008 Nov 18.
Many traditional Indian medicinal plants which contain large quantity of a secoiridoid, swertiamarin are being used to relieve pain. Iridoids present in a wide variety of medicinal plants possess a large number of medicinal properties. In the present study in vivo antinociceptive activity of swertiamarin isolated from E. axillare was carried out using three different methods in mice. In the hot plate method, a significant increase in the latency period was observed for the treatment with swertiamarin at 100 and 200 mg/kg bw after 30 and 45 min. The percent protection observed after 45 min was 109.42, 147.42 and 157.14, respectively, for the standard paracetamol and swertiamarin at 100 and 200 mg/kg bw treatments. A significant increase in the tail withdrawal reflex was observed for the swertiamarin treatment at both the doses with percent protections of 150 and 200, respectively. In both these methods, swertiamarin produced potent activity than that of standard paracetamol. In the acetic acid induced writhing, swertiamarin at 100 and 200 mg/kg bw reduced the number of writhes significantly. Dose dependent results were observed in all the three methods and among the two doses, swertiamarin at 200 mg/kg bw showed potent activity. These results prove that swertiamarin possess both peripheral and central antinociceptive activity.
许多含有大量裂环烯醚萜类化合物獐牙菜苦苷的传统印度药用植物被用于缓解疼痛。多种药用植物中存在的环烯醚萜类化合物具有大量药用特性。在本研究中，使用三种不同方法在小鼠身上对从腋花獐牙菜中分离出的獐牙菜苦苷进行了体内抗伤害感受活性研究。在热板法中，在30分钟和45分钟后，观察到100和200毫克/千克体重的獐牙菜苦苷处理组的潜伏期显著增加。45分钟后，标准对乙酰氨基酚以及100和200毫克/千克体重的獐牙菜苦苷处理组观察到的保护百分比分别为109.42、147.42和157.14。两种剂量的獐牙菜苦苷处理组均观察到尾部退缩反射显著增加，保护百分比分别为150和200。在这两种方法中，獐牙菜苦苷产生的活性均强于标准对乙酰氨基酚。在醋酸诱导的扭体实验中，100和200毫克/千克体重的獐牙菜苦苷显著减少了扭体次数。在所有三种方法中均观察到剂量依赖性结果，在两种剂量中，200毫克/千克体重的獐牙菜苦苷显示出较强的活性。这些结果证明獐牙菜苦苷具有外周和中枢抗伤害感受活性。
