McCabe R, Meagher S, Mullins B
Medical Service, Martinez Veterans Administration Medical Center, California 94553.
Infect Immun. 1991 May;59(5):1633-8. doi: 10.1128/iai.59.5.1633-1638.1991.
To determine if exogenous gamma interferon is effective in immunosuppressed mice infected with Trypanosoma cruzi, recombinant murine gamma interferon was administered to cyclosporin-treated mice with either acute or chronic T. cruzi infection. Gamma interferon significantly decreased parasitemia and prevented death in acutely infected mice. Parasitemias and mortality of mice treated with both gamma interferon and cyclosporin were similar to those of immunocompetent controls. In chronically infected mice, cyclosporin treatment produced significantly more organ explant cultures positive for T. cruzi. Fewer positive cultures, particularly for spleen and heart, were obtained from cyclosporin-treated mice when they also received gamma interferon. Ketoconazole treatment of mice resulted in no positive cultures. Cyclosporin treatment did not prevent activation of peritoneal macrophages by parenteral gamma interferon, nor did it have a consistent effect on serum titers of alpha/beta or gamma interferon in response to a second challenge inoculum of T. cruzi. These data indicate that exogenous gamma interferon suppresses acute and chronic T. cruzi infection in cyclosporin-treated mice but that gamma interferon is not as effective as the relatively specific antimicrobial ketoconazole. Gamma interferon activates macrophages despite cyclosporin treatment, and its effects appear to be tissue specific.
为了确定外源性γ干扰素对感染克氏锥虫的免疫抑制小鼠是否有效，将重组鼠γ干扰素给予患有急性或慢性克氏锥虫感染且经环孢素处理的小鼠。γ干扰素显著降低了急性感染小鼠的寄生虫血症并预防了死亡。同时接受γ干扰素和环孢素治疗的小鼠的寄生虫血症和死亡率与免疫活性对照组相似。在慢性感染小鼠中，环孢素治疗使更多的器官外植体培养物检测出克氏锥虫呈阳性。当环孢素处理的小鼠同时接受γ干扰素时，获得的阳性培养物减少，尤其是脾脏和心脏的阳性培养物。酮康唑治疗的小鼠未出现阳性培养物。环孢素治疗既未阻止肠外γ干扰素对腹腔巨噬细胞的激活，也未对第二次接种克氏锥虫后α/β或γ干扰素的血清滴度产生一致的影响。这些数据表明，外源性γ干扰素可抑制环孢素处理小鼠的急性和慢性克氏锥虫感染，但γ干扰素不如相对特异性的抗微生物药物酮康唑有效。尽管进行了环孢素治疗，γ干扰素仍可激活巨噬细胞，且其作用似乎具有组织特异性。
