Yang Yu, Knust Steffen, Schwiderek Sabrina, Qin Qin, Yun Qing, Grundmeier Guido, Keller Adrian
Technical and Macromolecular Chemistry, Paderborn University, Warburger Str. 100, 33098 Paderborn, Germany.
Nanomaterials (Basel). 2021 Feb 1;11(2):357. doi: 10.3390/nano11020357.
The nanoscale surface topography of biomaterials can have strong effects on protein adsorption. While there are numerous surface statistical parameters for the characterization of nanorough surfaces, none of them alone provides a complete description of surface morphology. Herein, a selection of nanorough titanium oxide surfaces has been fabricated with root-mean-square roughness () values below 2.7 nm but very different surface morphologies. The adsorption of the proteins myoglobin (MGB), bovine serum albumin (BSA), and thyroglobulin (TGL) at these surfaces was investigated in situ by ellipsometry to assess the importance of six of the most common surface statistical parameters. For BSA adsorption, both protein film thickness and time constant of adsorption were found to scale linearly with s. For TGL, however, the same adsorption characteristics depend linearly on the surface skewness (), which we attribute to the rather extreme size of this protein. Finally, a mixed behavior is observed for MGB adsorption, showing different linear correlations with and . These results demonstrate the importance of a thorough morphological characterization of the surfaces employed in protein adsorption and possibly also cell adhesion studies.
生物材料的纳米级表面形貌会对蛋白质吸附产生强烈影响。虽然有许多用于表征纳米粗糙表面的表面统计参数，但没有一个能单独完整描述表面形态。在此，制备了一系列纳米粗糙的二氧化钛表面，其均方根粗糙度（）值低于2.7 nm，但表面形态差异很大。通过椭偏仪原位研究了肌红蛋白（MGB）、牛血清白蛋白（BSA）和甲状腺球蛋白（TGL）在这些表面的吸附情况，以评估六个最常见表面统计参数的重要性。对于BSA吸附，发现蛋白质膜厚度和吸附时间常数均与 呈线性比例关系。然而，对于TGL，相同的吸附特性与表面偏度（）呈线性相关，我们将此归因于该蛋白质相当大的尺寸。最后，观察到MGB吸附呈现混合行为，与 和 呈现不同的线性相关性。这些结果表明，在蛋白质吸附以及可能的细胞黏附研究中，对所用表面进行全面的形态表征非常重要。
