Yu Yuanyuan, Sun Gongping, Liu Guangyi, Wang Yingshuo, Shao Zhengping, Chen Zhimin, Yang Jun
The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310008, China.
Microb Pathog. 2009 Feb;46(2):63-72. doi: 10.1016/j.micpath.2008.10.014. Epub 2008 Nov 24.
The role of sphingolipids in bacterial pathogenesis has been gradually recognized. In an effort to identify the possible involvement of sphingolipids during Mycoplasma pneumoniae (M. pneumoniae) infection, we first adopted a lipidomic approach to achieve the profiles of major sphingolipid species of M. pneumoniae as well as human lung carcinoma A549 cells, and further evaluated the effects of M. pneumoniae infection on sphingolipid metabolism in A549 cells. It was shown that M. pneumoniae and A549 cells share many common sphingolipid species, however, M. pneumoniae possesses certain specific molecular species that are not found in A549 cells. On the other hand, M. pneumoniae infection could alter sphingolipid metabolism in A549 cell, including the generation of new ceramide and sphingomyelin species, or the increase/decrease of intensities, which varies depending on the different infection doses and times. The effects of M. pneumoniae infection on two key enzymes in sphingolipid metabolism, serine palmitoyltransferase (SPT) and acid sphingomyelinase (ASM), were also examined. It was found that M. pneumoniae infection could affect the expression of SPT or the distribution of ASM at certain concentrations. These data suggest that M. pneumoniae infection could influence sphingolipid metabolism of its host, which might be related to its pathogenicity.
鞘脂类在细菌致病过程中的作用已逐渐得到认可。为了确定鞘脂类在肺炎支原体（M. pneumoniae）感染过程中可能的参与情况，我们首先采用脂质组学方法来获取肺炎支原体以及人肺癌A549细胞中主要鞘脂类物质的概况，并进一步评估肺炎支原体感染对A549细胞中鞘脂类代谢的影响。结果表明，肺炎支原体和A549细胞共享许多常见的鞘脂类物质，然而，肺炎支原体拥有某些在A549细胞中未发现的特定分子种类。另一方面，肺炎支原体感染可改变A549细胞中的鞘脂类代谢，包括新神经酰胺和鞘磷脂种类的产生，或强度的增加/减少，这取决于不同的感染剂量和时间。还研究了肺炎支原体感染对鞘脂类代谢中两种关键酶，丝氨酸棕榈酰转移酶（SPT）和酸性鞘磷脂酶（ASM）的影响。发现肺炎支原体感染在一定浓度下可影响SPT的表达或ASM的分布。这些数据表明，肺炎支原体感染可影响其宿主的鞘脂类代谢，这可能与其致病性有关。
