School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Extremophiles. 2012 Nov;16(6):895-901. doi: 10.1007/s00792-012-0485-5. Epub 2012 Sep 30.
Two halophilic archaeal strains, R30(T) and tADL(T), were isolated from an aquaculture farm in Dailing, China, and from Deep Lake, Antarctica, respectively. Both have rod-shaped cells that lyse in distilled water, stain Gram-negative and form red-pigmented colonies. They are neutrophilic, require >120 g/l NaCl and 48-67 g/l MgCl(2) for growth but differ in their optimum growth temperatures (30 °C, tADL(T) vs. 40 °C, R30(T)). The major polar lipids were typical for members of the Archaea but also included a major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The 16S rRNA gene sequences of the two strains are 97.4 % identical, show most similarity to genes of the family Halobacteriaceae, and cluster together as a distinct clade in phylogenetic tree reconstructions. The rpoB' gene similarity between strains R30(T) and tADL(T) is 92.9 % and less to other halobacteria. Their DNA G + C contents are 62.4-62.9 mol % but DNA-DNA hybridization gives a relatedness of only 44 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe two new species of a novel genus, represented by strain R30(T) (= CGMCC 1.10593(T) = JCM 17270(T)) and strain tADL(T) (= JCM 15066(T) = DSMZ 22187(T)) for which we propose the names Halohasta litorea gen. nov., sp. nov. and Halohasta litchfieldiae sp. nov., respectively.
两株嗜盐古菌菌株，R30(T)和 tADL(T)，分别从中国大菱鲆养殖场和南极洲深海湖分离得到。它们的细胞均呈杆状，在蒸馏水中溶解，革兰氏染色阴性，形成红色色素菌落。它们是嗜中性的，生长需要 >120 g/l NaCl 和 48-67 g/l MgCl(2)，但最适生长温度不同（30°C，tADL(T) vs. 40°C，R30(T)）。主要极性脂类为古菌的典型脂类，但也包括一种主要的糖脂，色谱与硫酸甘露糖葡糖基二醚（S-DGD-1）相同。两株菌的 16S rRNA 基因序列相似度为 97.4%，与 Halobacteriaceae 家族的基因最为相似，并在系统发育树重建中聚类为一个独特的分支。菌株 R30(T)和 tADL(T)之间的 rpoB' 基因相似度为 92.9%，与其他嗜盐菌相似度较低。它们的 DNA G+C 含量为 62.4-62.9 mol%，但 DNA-DNA 杂交的相关性仅为 44%。基于表型、化学分类和系统发育特性，我们描述了一个新属的两个新种，以 R30(T)株（=CGMCC 1.10593(T)=JCM 17270(T)）和 tADL(T)株（=JCM 15066(T)=DSMZ 22187(T)）为代表，分别命名为 Halohasta litorea 属，新种和 Halohasta litchfieldiae 种。
