Lekic T, Tang J, Zhang J H
Department of Physiology and Pharmacology, Loma Linda University, Loma Linda, CA 92354, USA.
Acta Neurochir Suppl. 2008;105:131-4. doi: 10.1007/978-3-211-09469-3_27.
Approximately 15% of all strokes are due to intracerebral hemorrhage (ICH) and of these, 5-10% occur in the cerebellum. The resultant mortality is around 20-30%. However, there is no well-established animal model to address this important clinical problem. We induced intracerebellar hemorrhage in rats using stereotaxic collagenase injection through a burr-hole into right cerebellum. Dosage-dependent effect of collagenase (0.2, 0.4, and 0.6 U) was tested in male and female rats. Brain edema formation was assessed by brain water content and hemorrhagic volume measured by hemoglobin assay. Wire suspension, inclined plane, beam walking, and neurological deficit score assessed neurological outcome. Marked hematoma was observed in right cerebellum, accompanied by brain edema in a dose-related fashion. When comparing sexes, hemorrhagic volume and neurological deficit scores were significantly increased in females compared to male counterpart. Females had mortality of 16%, while there was no mortality in male rats. Neurological deficits assessed by both beam walking and inclined plane were significantly increased at 0.4 and 0.6 U in females, but only at 0.6 for males. This new cerebellar hemorrhage rat model demonstrated dosage- and sex-dependent changes in hemorrhagic volume, brain edema, and neurological deficits, and could be used to test treatment strategies for ICH.
所有中风病例中约15%是由脑出血（ICH）引起的，其中5 - 10%发生在小脑。由此导致的死亡率约为20 - 30%。然而，目前尚无完善的动物模型来解决这一重要的临床问题。我们通过立体定位将胶原酶经颅骨钻孔注射到大鼠右侧小脑，诱导大鼠发生小脑出血。在雄性和雌性大鼠中测试了胶原酶（0.2、0.4和0.6单位）的剂量依赖性效应。通过脑含水量评估脑水肿形成情况，并通过血红蛋白测定法测量出血体积。通过悬线试验、斜面试验、横梁行走试验和神经功能缺损评分评估神经功能结局。在右侧小脑中观察到明显的血肿，并伴有与剂量相关的脑水肿。比较不同性别时，雌性大鼠的出血体积和神经功能缺损评分相比雄性大鼠显著增加。雌性大鼠的死亡率为16%，而雄性大鼠无死亡。在雌性大鼠中，通过横梁行走试验和斜面试验评估的神经功能缺损在胶原酶剂量为0.4和0.6单位时显著增加，而在雄性大鼠中仅在0.6单位时显著增加。这种新的小脑出血大鼠模型表现出出血体积、脑水肿和神经功能缺损的剂量和性别依赖性变化，可用于测试脑出血的治疗策略。
