来自海洋链霉菌属菌株M491的T-穆罗洛尔倍半萜以及对先前报道的无定形烷构型的修订

T-muurolol sesquiterpenes from the marine Streptomyces sp. M491 and revision of the configuration of previously reported amorphanes.

作者信息

Ding Ling, Pfoh Roland, Rühl Stephan, Qin Song, Laatsch Hartmut

机构信息

Department of Organic and Biomolecular Chemistry, University of Göttingen, Tammannstrasse 2, D-37077 Göttingen, Germany.

出版信息

J Nat Prod. 2009 Jan;72(1):99-101. doi: 10.1021/np8006843.

DOI:10.1021/np8006843

PMID:19072130

Abstract

Two new sesquiterpenes, 15-hydroxy-T-muurolol (3d) and 11,15-dihydroxy-T-muurolol (3e), along with the plant cadinenes T-muurolol (3f) and 3alpha-hydroxy-T-muurolol (3g), were isolated from the marine-derived Streptomyces sp. M491. Their absolute configuration was established via NMR spectroscopy and X-ray crystallography of 3-oxo-T-muurolol (3a), which was reisolated from this strain. In addition, the absolute configuration of further sesquiterpenes previously reported from this strain was revised. These products were tested for their cytotoxicity against 37 human tumor cell lines using the MTT method. Only 3d was cytotoxic against a range of human tumor cell lines with a mean IC50 of 6.7 microg/mL.

摘要

从海洋来源的链霉菌属菌株M491中分离出两种新的倍半萜，15-羟基-T-桉叶醇（3d）和11,15-二羟基-T-桉叶醇（3e），以及植物杜松烯类T-桉叶醇（3f）和3α-羟基-T-桉叶醇（3g）。通过从该菌株中重新分离得到的3-氧代-T-桉叶醇（3a）的核磁共振光谱和X射线晶体学确定了它们的绝对构型。此外，对先前报道的来自该菌株的其他倍半萜的绝对构型进行了修正。使用MTT法测试了这些产物对37种人类肿瘤细胞系的细胞毒性。只有3d对一系列人类肿瘤细胞系具有细胞毒性，平均IC50为6.7μg/mL。