采用气相色谱/质谱联用技术测定脱落酸和赤霉素。
Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry.
作者信息
Okamoto Masanori, Hanada Atsushi, Kamiya Yuji, Yamaguchi Shinjiro, Nambara Eiji
机构信息
Growth Regulation Research Group, RIKEN Plant Science Center, Tsurumi, Yokohama, Japan.
出版信息
Methods Mol Biol. 2009;495:53-60. doi: 10.1007/978-1-59745-477-3_5.
Gas chromatography-mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.
基于气相色谱-质谱联用（GC-MS）的分析是一种准确且灵敏的植物激素定量方法。该方法常用于分析低分子量化合物，如脱落酸（ABA）、赤霉素（GAs）、生长素和油菜素甾体类化合物。实验步骤主要包括四个主要环节：提取、分馏、衍生化和检测。在此，我们描述一种ABA和GAs定量分析的实验方案。
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