Kovary K, Bravo R
Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.
Mol Cell Biol. 1991 Sep;11(9):4466-72. doi: 10.1128/mcb.11.9.4466-4472.1991.
The expression of different members of the Jun and Fos families of transcription factors is rapidly induced following serum stimulation of quiescent fibroblasts. To determine whether these proteins are required for cell cycle progression, we microinjected affinity-purified antibodies directed against c-Fos, FosB, Fra-1, c-Jun, JunB, and JunD, and antibodies that recognize either the Fos or the Jun family of proteins, into Swiss 3T3 cells and determined their effects in cell cycle progression by monitoring DNA synthesis. We found that microinjection of anti-Fos and anti-Jun family antibodies efficiently blocked the entrance to the S phase of serum-stimulated or asynchronously growing cells. However, the antibodies against single members of the Fos family only partially inhibited DNA synthesis. In contrast, all three Jun antibodies prevented DNA synthesis more effectively than did any of the anti-Fos antibodies.
在血清刺激静止的成纤维细胞后，转录因子Jun和Fos家族不同成员的表达会迅速被诱导。为了确定这些蛋白质是否是细胞周期进程所必需的，我们将针对c-Fos、FosB、Fra-1、c-Jun、JunB和JunD的亲和纯化抗体，以及识别Fos或Jun蛋白家族的抗体显微注射到瑞士3T3细胞中，并通过监测DNA合成来确定它们对细胞周期进程的影响。我们发现，显微注射抗Fos和抗Jun家族抗体可有效阻断血清刺激或异步生长细胞进入S期。然而，针对Fos家族单个成员的抗体仅部分抑制DNA合成。相比之下，所有三种Jun抗体比任何一种抗Fos抗体更有效地阻止了DNA合成。
