甲型/ H3N2流感病毒M2和血凝素基因的体内和体外变化：在MDCK - SIAT1细胞和传统MDCK细胞中传代的影响
In vivo and in vitro alterations in influenza A/H3N2 virus M2 and hemagglutinin genes: effect of passage in MDCK-SIAT1 cells and conventional MDCK cells.
作者信息
Li Danjuan, Saito Reiko, Suzuki Yasushi, Sato Isamu, Zaraket Hassan, Dapat Clyde, Caperig-Dapat Isolde Ma, Suzuki Hiroshi
机构信息
Department of Public Health, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.
出版信息
J Clin Microbiol. 2009 Feb;47(2):466-8. doi: 10.1128/JCM.00892-08. Epub 2008 Dec 17.
Abstract
No mutations were detected in the hemagglutinin gene of influenza A/H3N2 virus isolates from patients undergoing short-term amantadine treatment. However, genetic changes occurred after serial passage in either MDCK or MDCK-SIAT1 cells. Our results showed that only a few mutations were observed in MDCK-SIAT1-passaged isolates in the presence of amantadine.
摘要
在接受短期金刚烷胺治疗的患者所分离出的甲型H3N2流感病毒的血凝素基因中未检测到突变。然而，在MDCK或MDCK-SIAT1细胞中连续传代后发生了基因变化。我们的结果表明，在存在金刚烷胺的情况下，在MDCK-SIAT1传代分离株中仅观察到少数突变。
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