猪早期妊娠和黄体溶解期间子宫内膜和肌层雄激素及雌酮的分泌

Endometrial and myometrial secretion of androgens and estrone during early pregnancy and luteolysis in pigs.

作者信息

Franczak Anita

机构信息

Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-718 Olsztyn, Poland.

出版信息

Reprod Biol. 2008 Nov;8(3):213-28. doi: 10.1016/s1642-431x(12)60013-8.

DOI:10.1016/s1642-431x(12)60013-8

PMID:19092984

Abstract

UNLABELLED

Previously, we found that in addition to embryos, the uterine tissues may be a source of estradiol-17beta (E(2)) during early pregnancy in the pig. The aim of the present study was to determine whether porcine endometrium and myometrium secrete androgens - androstenedione (A(4)), testosterone (T) and estrone (E(1)) during early pregnancy and luteolysis (Days 14-16) in pigs. Individual endometrial and myometrial slices (200 mg) were first pre-incubated (24 h) and then incubated (6 h, 37 degrees C in an atmosphere of 95% O(2) and 5% CO(2)) in the presence or absence of progesterone (P(4); 10(-5) M), oxytocin (OT; 10(-7) M) or OT plus P(4). Basal endometrial and myometrial secretion of A(4) and T did not differ between pregnant and cyclic gilts. Endometrial secretion of E(1) was higher in pregnant than cyclic gilts (p<0.05) while myometrial secretion of E(1) did not differ between the two groups of the examined pigs (p>0.05). Progesterone significantly increased A(4) and T secretion (p<0.001) by uterine tissues regardless of the reproductive status. In the presence of P(4), endometrial and myometrial secretion of E(1) was increased only during luteolysis (p<0.001). In both tissues, OT did not affect the examined steroid secretion and did not change the effect of P(4).

IN CONCLUSION

porcine endometrium and myometrium was found to produce A(4), T and E(1) in vitro; 2) basal endometrial and myometrial production of A(4) and T did not differ between the examined reproductive periods; 3) the endometrium released more E(1) during early pregnancy than luteolysis; 4) in the presence of substrate (P(4)), uterine tissues increased secretion of A(4) and T during early pregnancy and luteolysis; and 5) P(4) increased uterine production of E(1) only during luteolysis. These data demonstrated the presence of the active steroid pathway in porcine endometrium and myometrium which may serve as an alternative source of androgens and estrogens in pigs.

摘要

未标记

此前，我们发现，在猪的妊娠早期，除胚胎外，子宫组织可能是雌二醇-17β（E₂）的来源。本研究的目的是确定猪子宫内膜和子宫肌层在妊娠早期和黄体溶解期（第14 - 16天）是否分泌雄激素——雄烯二酮（A₄）、睾酮（T）和雌酮（E₁）。首先将单个子宫内膜和子宫肌层切片（200毫克）预孵育（24小时），然后在有或无孕酮（P₄；10⁻⁵M）、催产素（OT；10⁻⁷M）或OT加P₄的情况下孵育（6小时，37℃，95% O₂和5% CO₂气氛）。妊娠母猪和发情周期母猪的子宫内膜和子宫肌层基础分泌的A₄和T没有差异。妊娠母猪子宫内膜分泌的E₁高于发情周期母猪（p<0.05），而两组受试猪子宫肌层分泌的E₁没有差异（p>0.05）。无论生殖状态如何，孕酮均显著增加子宫组织分泌的A₄和T（p<0.001）。在有P₄存在的情况下，仅在黄体溶解期子宫内膜和子宫肌层分泌的E₁增加（p<0.001）。在两种组织中，OT均不影响所检测的类固醇分泌，也不改变P₄的作用。

结论

1）发现猪子宫内膜和子宫肌层在体外可产生A₄、T和E₁；2）受试生殖期之间，子宫内膜和子宫肌层基础分泌的A₄和T没有差异；3）妊娠早期子宫内膜释放的E₁比黄体溶解期多；4）在有底物（P₄）存在的情况下，子宫组织在妊娠早期和黄体溶解期增加A₄和T的分泌；5）P₄仅在黄体溶解期增加子宫分泌的E₁。这些数据表明猪子宫内膜和子宫肌层中存在活跃的类固醇途径。该途径可能是猪雄激素和雌激素的另一个来源。