Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, China.
Front Public Health. 2022 Apr 19;10:865605. doi: 10.3389/fpubh.2022.865605. eCollection 2022.
Murine (MuKV) is a novel picornavirus of the genus , and was first identified in the feces of murine rodents in the USA in 2011. There is limited information on the transmission route of MuKV. Thus, we conducted a study to investigate virus detection rates in fecal, serum, throat, and lung tissue samples from murine rodents.
A total of 413 fecal samples, 385 lung samples, 269 throat swab samples, and 183 serum samples were collected from 413 murine rodents (, and ) captured in urban Shenzhen. Kobuviruses were detected via RT-PCR. Only fecal samples were positive, with prevalence rates of 34.9% in and 29.4% in . Phylogenetic analysis based on partial 3D and complete VP1 sequence regions indicated that all of the MuKV sequences obtained belonged to , and were genetically closely related to other MuKVs reported in China, Hungary, and the USA. Twenty-eight full-length MuKV sequences were acquired. Phylogenetic analysis of two sequences randomly selected from the two species (SZ59 and SZ171) indicated that they shared very high nucleotide and amino acid identity with one another (94.0 and 99.3%, respectively), and comparison with human revealed amino acid identity values of ~80%. Additionally, a sewage-derived sequence shared high similarity with the rat-derived sequences identified in this study, with respective nucleotide and amino acid identity values from 86.5 and 90.7% to 87.2 and 91.1%.
The results of the current study provide evidence that murine is transmitted via the fecal-oral route.
鼠诺如病毒（MuKV）是杯状病毒科 的一种新型小核糖核酸病毒，于 2011 年首次在美国啮齿动物的粪便中被发现。目前有关 MuKV 的传播途径知之甚少。因此，我们开展了一项研究，以调查从啮齿动物的粪便、血清、咽喉和肺组织样本中检测到的病毒。
从在深圳市捕获的 413 只啮齿动物（包括 、和 ）中采集了 413 份粪便样本、385 份肺样本、269 份咽喉拭子样本和 183 份血清样本。通过 RT-PCR 检测到科博病毒。只有粪便样本呈阳性，在 和 中的流行率分别为 34.9%和 29.4%。基于部分 3D 和完整 VP1 序列区域的系统进化分析表明，获得的所有 MuKV 序列均属于 ，与中国、匈牙利和美国报道的其他 MuKV 密切相关。共获得 28 条全长 MuKV 序列。对从两个物种（SZ59 和 SZ171）中随机选择的两个序列进行的系统进化分析表明，它们彼此之间具有非常高的核苷酸和氨基酸同一性（分别为 94.0%和 99.3%），与人类的氨基酸同一性值约为 80%。此外，从污水中获得的序列与本研究中鉴定的大鼠衍生序列高度相似，核苷酸和氨基酸的同一性值分别为 86.5%和 90.7%，至 87.2%和 91.1%。
本研究结果提供了证据表明，鼠诺如病毒通过粪-口途径传播。
