Schroeder Hendrik, Adler Michael, Gerigk Katrin, Müller-Chorus Birgit, Götz Friedrich, Niemeyer Christof M
Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto Hahn Str. 6, 44227 Dortmund, Germany.
Anal Chem. 2009 Feb 1;81(3):1275-9. doi: 10.1021/ac802228k.
We present a microfluidic device for multiplexed immunoassays based on DNA-directed immobilization. Because of the versatile building blocks used for this technique, it is possible to build up user configurable protein microarrays within the microfluidic system by means of DNA-directed self-assembly, which can be used for immunoassay applications. We demonstrate the performance of our system by parallel detection of cytokines in a multiplex immunoassay, employing silver deposition labeling and optical read-out.
我们展示了一种基于DNA定向固定的用于多重免疫测定的微流控装置。由于该技术使用了通用的构建模块，因此可以通过DNA定向自组装在微流控系统内构建用户可配置的蛋白质微阵列，其可用于免疫测定应用。我们通过在多重免疫测定中并行检测细胞因子来展示我们系统的性能，采用银沉积标记和光学读出。
